Protocols for Gene Transfer in Neuroscience
Towards Gene Therapy of Neurological Disorders
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|Format:||Paperback, 446 pages|
|Other Information: ||index|
|Published In: ||United Kingdom, 26 April 1996|
This is a study of the latest techniques used by gene delivery laboratories. The methodology under discussion includes physical transfection techniques, various viral vectors, and retrovirally-transduced cell and bone marrow transplantation. Several variations of a single protocol are given, thus demonstrating how different labs have solved a common problem in different ways. The protocols are also indexed and cross-referenced so that an investigator can find all the details required for a particular problem.
Table of Contents
PROTOCOLS FOR GENE DELIVERY TO CULTURED CELLS USING PHYSICAL METHODS: Gene Therapy into Post-Mitotic Neurons in Culture by Microinjection; PROTOCOLS FOR GENE DELIVERY USING RNA VIRUSES: Semliki Forest Virus Vectors for Gene Transfer; PROTOCOLS FOR GENE DELIVERY USING ADENOVIRUSES AND ADENO-ASSOCIATED VIRUSES: Adeno-Associated Virus Vectors for Gene Transfer; PROTOCOLS FOR GENE DELIVERY USING HERPES SIMPLEX VIRUS 1 VECTORS: Transcriptional Regulation of Herpes Simplex Virus Gene Expression: Implications for our Understanding of Latency; PROTOCOLS FOR GENE DELIVERY USING CELLS: Murine Mucopolysaccharides Type VII: A Model System for Somatic Gene Therapy of the Central Nervous System; PROTOCOLS FOR IN VIVO APPLICATION OF GENE TRANSFER METHODS: Replication-Competent Viral Therapy of Tumours in the Central Nervous System; PROSPECTS AND SHORTCOMINGS OF NEUROLOGICAL GENE THERAPY.
|Publisher: ||John Wiley & Sons Ltd|
|Dimensions: ||24.0 x 21.0 x 3.0 centimeters (1.03 kg)|