Table of Contents

List of Contributors.
Preface.
Introduction.
Historical Perspective.

PART ONE-BASIC BIOLOGICAL PROCESES
Activation of Mammalian Oocytes.
The Nucleus.
Nuclear Reprogramming: Biological and Technological Constraints.
Plasticity of somatic nucleus by epigenetic reprogramming via cell Hybridization.
Cell Cycle.
Determinants of Pluripotency in Mammals.
Cloning and Aging.

PART TWO-METHODS
Micromanipulation Techniques for Cloning.
Microinsemination and Nuclear Transfer with Male Germ Cells.
Development of viable mammalian embryos in vitro: Evolution of sequential media.
Genetic and Phenotypic Similarity Among Members of Mammalian Clonal Sets.
Genetic Modification and Cloning in Mammals.
Pregnancy and Neonatal Care of Clone Animals.
Donor Cell Type and Cloning Efficiency in Mammals.

PART THREE-CLONING BY SPECIES
The Cloning of Amphibians.
Cloning of Fish.
Cloning of Mice.
Cloning of Rabbits.
Nuclear Transfer in Swine.
Cloning of Cattle.
Cloning of Sheep.
Cloning of Goats.

PART FOUR-CURRENTLY SOUGHT AFTER SPECIES
Cloning of Endangered Species.
Cloning of Rats.

PART FIVE-NUCLEAR TRANSFER IN PRIMATES
Cloning in Non-Human Primates.

PART SIX-APPLICATIONS
Nuclear Transfer for Stem Cells (CRNT).
Current Research and Commercial Applications of Cloning Technology.
Transgenic Cloned Goats and the Production of Therapeutic Proteins.

PART SEVEN-ETHICAL AND LEGAL AFFAIRS
Ethical Implications of Cloning.

FINAL REMARKS
Mammalian Cloning- Challenges for the Future.

Promotional Information

Key Features
* First and most comprehensive book on animal cloning
* Chapters written by the world' expert in each area
* From the early experiments in amphibia to the latest one in mammals, everything is included in this book and told by the researcher that did it and how they did it
* Basic biological mechanisms on how cloning works and all their current and potential applications
* Cloning applications on basic biology, agriculture, biotechnology and medicine are included
* Editors are the pioneers in the field

About the Author

Dr. Cibelli is Professor of Animal Biotechnology at Michigan State University. He heads the Cellular Reprogramming Laboratory in the Departments of Animal Science and Physiology since 2003. From 2010 to 2017 he was also the Scientific Director of LARCel, a laboratory of cellular reprogramming dedicated to generating human pluripotent cells under GMP conditions for preclinical studies in Andalucia, Spain. Dr. Cibelli is internationally recognized as one of the pioneers in the area of cellular reprogramming using oocyte-driven protocols. Dr. Cibelli together with his colleagues, were responsible for the generation of the world's first transgenic cloned calves, the first stem cells by nuclear transfer in bovine, the first embryonic stem cells by parthenogenesis in primates and the generation of the first cell line of iPSCs using oocyte factors alone. His work has been published un numerous scientific journals including Science, Nature Biotechnology, Nature Medicine, Nature Methods, PNAS, Cell Stem Cell and JAMA. Robert Lanza is an American scientist and author whose research spans the range of natural science, from biology to theoretical physics. TIME magazine recognized him as one of the “100 Most Influential People in the World,” and Prospect magazine named him one of the Top 50 “World Thinkers.” He has hundreds of scientific publications and over 30 books, including definitive references in the fields of stem cells, tissue engineering, and regenerative medicine. He’s a former Fulbright Scholar and studied with polio-pioneer Jonas Salk and Nobel laureates Gerald Edelman (known for his work on the biological basis of consciousness) and Rodney Porter. He also worked closely (and co-authored papers in Science on self-awareness and symbolic communication) with noted Harvard psychologist BF Skinner. Dr. Lanza was part of the team that cloned the world’s first human embryo, the first endangered species, and published the first-ever reports of pluripotent stem cell use in humans.