The Major Histocompatibility Complex - A Paradigm for Studies of the Human Genome.- HLA Typing by SSO and SSP Methods.- Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity.- HLA Typing Using Bead Based Methods.- HLA Typing by Direct DNA Sequencing.- Data Analysis of HLA Sequencing using Assign-SBT v3.6+ from Conexio.- Simple Methods for the Detection of HLA-G Variants in Coding and Non-Coding Regions.- Molecular Typing of HLA-E.- Molecular Analysis of Complement Component C4 Gene Copy Number.- Genotyping of Single Nucleotide Polymorphisms (SNPs) by 5’ Nuclease Allelic Discrimination.- High Resolution MICA Genotyping by Sequence-Based Typing (SBT).- Standard Methods for the Management of Immunogenetic Data.- Analytical Methods for Immunogenetic Population Data.- Analytical Methods for Disease Association Studies with Immunogenetic Data.- Impact of HLA Matching and HLA Antibodies in Organ Transplantation: a Collaborative Transplant Study View.- Screening for Antibodies Against MICA by Luminex Flow Cytometry.- HLA Antibody Detection and Characterization by Solid Phase Immunoassays - Methods and Pitfalls.- Detection and Characterisation of Alloreactive T Cells.- Detection of Allo-HLA Crossreactivity by Viral Specific Memory T-Cell Clones using Single HLA Transfected K562 Cells.- Separation and Cryopreservation of Lymphocytes from Spleen and Lymph Node.- Crossmatching by Complement-Dependent Lymphocytotoxicity (CDC).- The Lymphocyte Crossmatch by Flow Cytometry for Kidney Transplantation.- Overview of the Killer Cell Immunoglobulin-Like Receptor (KIR) System.- KIR Typing by Non Sequencing Methods: Polymerase-Chain Reaction with Sequence-Specific Primers (PCR-SSP).- Killer Cell Immunoglobulin-Like Receptors (KIR) Typing by DNA Sequencing.- An Overview of Methods Required to Evaluate Donor NK Cell Alloreactivity for Haploidentical Haemopoietic Stem Cell Transplantation.- The Detection of NK Cell Alloreactivity by Flow CytometricCD107a Assay.- Clinical Production and Therapeutic Applications of Alloreactive Natural Killer Cells.- Minor Histocompatibility Antigen Typing by DNA Sequencing for Clinical Practice in Hematopoietic Stem-Cell Transplantation.- Donor Registries and Search Strategies.- Cytokine Gene Polymorphisms: Methods of Detection and Biological Significance.- IMGT® Tools for the Nucleotide Analysis of Immunoglobulin (IG) and T Cell Receptor (TR) V-(D)-J Repertoires, Polymorphisms, and IG Mutations: IMGT/V-QUEST and IMGT/HighV-QUEST for NGS.- IMGT/DomainGapAlign: the IMGT® Tool for the Analysis of IG, TR, MH, IgSF and MhSF Domain Amino Acid Polymorphism.- Human Gm, Km and Am Allotypes and their Molecular Characterization: a Remarkable Demonstration of Polymorphism.
From the reviews:“This book is a comprehensive volume covering a wide-range of topics related to HLA in the clinical setting. … this book is an extremely useful reference for those working in the field of transplantation, or with an interest in the clinical applications of HLA. The introductory chapters to various topics are clear and provide a good amount of detail and are well referenced for those who wish to read further on the subject.” (Vivien Hanson, Immunology News, February, 2013)
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