Table of Contents

Part 1: Structure and Function of Enzymes 1. An Introduction to Enzymes 2. The Structure of Proteins 3. The Biosynthesis and Properties of Proteins 4. Specificity of Enzyme Action 5. Monomeric and Oligomeric Enzymes

Part 2: Kinetic and Chemical Mechanisms of Enzyme-Catalysed Reactions 6. An Introduction to Bioenergetics, Catalysis and Kinetics 7. Kinetics of Single-Substrate Enzyme-Catalysed Reactions 8. Enzyme Inhibition 9. Kinetics of Multi-Substrate Enzyme-Catalysed Reactions 10. The Investigation of Active Site Structure 11. The chemical nature of enzyme catalysis 12. The Binding of Ligands to Proteins 13. Sigmoidal Kinetics and Allosteric Enzymes 14. The Significance of Sigmoidal Behaviour

Part 3: Application of Enzymology 15. Investigation of Enzymes in Biological Preparations 16. Extraction and Purification of Enzymes 17. Enzymes as Analytical Reagents 18. Instrumental Techniques Available for Use in Enzymatic Analysis 19. Applications of Enzymatic Analysis in Medicine, Forensic Science and Industry 20. Biotechnological Applications of Enzymes 21. Genomics, Proteomics and Bioinformatics

About the Author

Trevor Palmer and Philip L Bonner, Nottingham Trent University, UK

Reviews

Review of the first edition: "Essential. ...explains the structure and function of enzymes. …every chapter tells of an advance in understanding proteins or their interactions. I will definitely use this book." --Chemistry and Industry (Professor Carole Hackney, Keele University, UK)

"Outstandingly written, updated on molecular biology and bioinformatics, with particularly good treatment of steady-state enzyme kinetics and analytical applications. For undergraduates in biochemistry, biological and analytical sciences, and medicine." --Natural Products Report (Royal Society of Chemistry)

"An excellent book, warmly recommended. --Trends in Biotechnology