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Part 1. Overview of Astrocyte Biology 1. Astrocytes: Multi-talented Stars of the Central Nervous System(Ransom-B) 2. Neurogenic Astrocytes and their Glycoconjugates: not just "Glue" Anymore (Steindler-D) 3. Heterogeneity of Astrocytic Form and Function (Goldman-S/ Nedergaard-M) Part 2. Isolation and Culture of Astrocytes4. Preparation of Mixed Glial Cultures from Postnatal Rat Brain(DeVellis-J) 5. Generation of Primary Astrocyte Cultures Devoid of Contaminating Microglia (Hewett-S 6. Derivation of Microglia-free Astrocyte Cultures from Neural Stem Cells (Milner-R) 7. Isolation and Culture of Spinal Cord Astrocytes (Miller-R 8. Isolation and Culture of Astrocytes from the Retina and Optic Nerve (Lukas-T) 9. Primary Cultures of Astrocytes from Fetal Bovine Brain (Ballarin-C) 10. Isolation and Cultivation of Porcine Astrocytes (Bobilya-D) 11. Isolation and Culture of Human Astrocytes (Sharif-A/ Prevot-V) 12. Cell Culturing of C. elegans Glial Cells for the Assessment of Cytosolic Ca2+ Dynamics (Parpura-V) Part 3. Analysis of Astrocyte Functions In Vitro 13. Astrocyte Polarization and Wound Healing in Culture:Studying Cell Adhesion Molecules (Carbonetto-S) 14. An in Vitro Trauma Model to Study Rodent and Human Astrocyte Reactivity (Wanner-I) 15. Use of Gel Zymography to Examine Matrix Metalloproteinase (gelatinase) Expression in Brain Tissue or in Primary Glial Cultures (del Zoppo-G) 16. ELISA Methodology to Quantify Astrocyte Production of Cytokines/Chemokines In Vitro (Benveniste-T) 17. Induction of Nitric Oxide Synthase-2 Expression and Measurement of Nitric Oxide Production in Enriched Primary Cortical Astrocyte Cultures (Hewett-J) 18. Examining Potassium Channel Function in Astrocytes (Olsen-M) 19. Connexin Based Channels in Astrocytes: How to Study their Properties (Giaume-C) 20. Isotope-based Quantitation of Uptake, Release and Metabolism of Glutamate and Glucose in Cultured Astrocytes (Hertz-L) Part 4. Co-culture systems to analyse astrocyte interactions with other cell types21. An in Vitro Model of Reactive Astrogliosis and its Effect on Neuronal Growth (Geller-H) 22. A Neuron-Astrocyte Co-Culture System to Investigate Astrocyte Secreted Factors in Mouse Neuronal Development (Murai-K) 23. Co-cultures of Neurons and Astrocytes as a Model for Examining Hypoxia-induced Neuronal Death (Rempe-D). 24. Use of Astrocyte-Microglial Co-cultures to Examine the Regulatory Influence of Astrocytes on Microglial Activation (Milner-R) 25. Astrocyte-Schwann Cell Co-Culture Systems (Fawcett-J) 26. An in Vitro Assay to Examine Oligodendrocyte Precursor Cell Migration on Astrocytes (Fawcett-J) 27. Investigation of Astrocyte-Oligodendrocyte Interactions in Human Cultures (John-G) 28. An Improved in vitro Blood-Brain Barrier Model: Rat Brain Endothelial Cells Co-Cultured with Astrocytes (Abbott-J) 29. In Vitro Models of the Blood-Brain Barrier (Abbruscato-T) 30. An in Vitro Model of Ischemic Stroke (Abbruscato-T) 31. Pericyte Co-Culture Models to Study Astrocyte, Pericyte, and Endothelial Cell Interactions (Dore-Duffy-P) Part 5. Analysis of Astrocyte Functions In Vivo32. Reprogramming of Postnatal Astroglia of the Mouse Neocortex into Functional Synapse-Forming Neurons (Berninger-B) 33. Assessment of Glial Function in the in Vivo Retina (Newman-E) 34. Imaging in Vivo Astrocyte/Endothelial Cell Interactions at the Blood-Brain Barrier (Willis-C) 35. Transgenic Techniques for Cell Ablation or Molecular Deletion to Investigate Functions of Astrocytes and Other GFAP-Expressing Cell Types (Sofroniew-M) 36. Use of Cre-Lox Technology to Analyze Integrin Functions in Astrocytes (McCarty-J) 37. In Vivo Imaging of Ca2+ Signaling in Astrocytes Using Two Photon Laser Scanning Fluorescent Microscopy (Ding-S)

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