Table of Contents

Acknowledgments
How To Use This Manual
PART I: AN OVERVIEW OF BACULOVIRUSES
1: Virus Structure and the Infection Process
2: Gene Organization, Regulation, and Function
3: Virus-Host Interactions
4: Summary of Baculovirus Features Relevant to Expression Factors
PART II: CHOOSING A TRANSFER PLASMID AND PARENT VIRUS
5: Choice of Virus and Host Species
6: Choice of Transfer Plasmid
7: Available Transfer Plasmids
8: Choosing a Parent Virus for Use in Vector Construction
9: Optimizing Expression: Tailoring the Heterologous Gene to the Transfer Plasmid and the Baculovirus Expression System
PART III: METHODS FOR VECTOR CONSTRUCTION AND GENE EXPRESSION
10: Safety Considerations
11: Insect Cell Culture
12: Virus Methods
13: Contransfection and Recombinant Virus Identification
14: Characterizing Recombinant Gene Expression
15: Post-Translational Modification
PART IV: METHODS FOR SCALE-UP OF PROTEIN PRODUCTION AND USE OF INSECT LARVAE
16: David L. Clemm: Scale-Up of Protein Production in a Stirred Bioreactor
17: Cheryl Isaac Murphy: Scale-Up of Protein Production in an Airlift Fermenter
18: Insect Rearing and Infection
Appendix 1: Nucleotide Sequence of AcMNPV Polyhedrin Region
Appendix 2: Nucleotide Sequence of AcMNPV p10 Region
Appendix 3: List of Genes Expressed in the Baculovirus Expression System
Appendix 4: Suppliers
Appendix 5: Stock Solutions
Appendix 6: Excel Spreadsheet for TCID[5[0 Calculation
References
Index

Reviews

"A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et
al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work." --Cell
"Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview
prior to taking the plunge." --Tibtech
"A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et
al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work." --Cell
"Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview
prior to taking the plunge." --Tibtech