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Carbohydrate Biotechnology Protocols (Methods in Biotechnology)

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Carbohydrate Biotechnology Protocols

Methods in Biotechnology

By Christopher Bucke (Edited by)

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Format: Paperback, 337 pages, 1st ed. Softcover of Edition
Other Information: biography
Published In: United States, 01 November 2010
A compilation of modern hands-on methods using microbes and enzymes to produce and modify carbohydrates of potential and actual commercial value. These powerful methods enable both the expert and the beginner to generate polysaccharides, oligosaccharides, and carbohydrate-based surfactants by fermentation using enzymes. Additional techniques make it possible to produce derivatives of sugars, other oligosaccharides, and sugar derivatives using enzyme technology. Carbohydrate Biotechnology Protocols offers synthetic chemists, biochemists, fermentation technologists, and applied enzymologists cutting-edge techniques-many of them hitherto unavailable-that are cleaner and often less costly than available chemical alternatives.

Table of Contents

Introduction to Carbohydrate Biotechnology, Christopher Bucke. Production and Isolation of Xanthan Gum, Felix Garcia-Ochoa, Victoria E. Santos, and Jose A. Casas. Alginate from Azotobacter vinelandii, Francesca Clementi, Mauro Moresi, and Eugenio Parente. Production of Schizophyllan, Udo Rau. Enzymatic Synthesis of Cellulose, Shiro Kobayashi, Shin-ichiro Shoda, Michael J. Donnelly, and Stephen P. Church. Modification of Alginate Using Mannuronan C-5-Epimerases, Helga Ertesvag and Gudmund Skjak-Braek. Viscosity Control of Guar Polysaccharide Solutions by Treatment with Galactose Oxidase and Catalase Enzymes, Michael J. Donnelly. The Production of Cyclodextrins Using CGTase from Bacillus macerans, Jacob A. Rendleman, Jr. Production of Microbial Glycolipids, Siegmund Lang. Partial Enzymatic Hydrolysis of Starch to Maltodextrins on the Laboratory Scale, Leon M. Marchal. The Production of a(1AE2)-Terminated Glucooligosaccharides, Marguerite Dols, Vincent Monchois, Magali Remaud-Simeon, Rene-Marc Willemot, and Pierre F. Monsan. Enzymatic Production of Fructooligosaccharides from Sucrose, Jong Won Yun and Seung Koo Song. Enzymatic Production of Inulooligosaccharides from Inulin, Jon Won Yun and Dong Hyun Kim. One-Pot Enzymatic Synthesis of Sialyl T-Epitope, Vladimir Kren. Hydrolysis of Hemicelluloses Using Combinations of Xylanases and Feruloyl Esterases, Craig B. Faulds, Paul A. Kroon, Begona Bartolome, and Gary Williamson. Enzymatic Depolymerization of Chitins and Chitosans, Riccardo A. A. Muzzarelli, Vesna Stanic, and Viviana Ramos. Synthesis of Homo- and Hetero-Oligosaccharides from Underivatized Sugars Using Glycosidases, Christopher Bucke, John Packwood, Sony Suwasono, and Robert A. Rastall. Use of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE (R)) in the Elucidation of N-Linked Oligosaccharide Structures, Harish P. M. Kumar, Patricia A. Denny, and Paul C. Denny. Application of Sucrose Synthase in the Synthesis of Nucleotide Sugars and Saccharides,Astrid Zervosen and Lothar Elling. Production of Isomaltulose Using Immobilized Bacterial Cells, Peter S. J. Cheetham and Christopher Bucke. The Production of Mannitol by Fermentation, Wim Soetaert, Petra T. Vanhooren, and Erick J. Vandamme. The Production of 3-Keto-Derivatives of Disaccharides, Eberhard Stoppok and Klaus Buchholz. Enzymatic Synthesis of a-Butylglucoside in a Biphasic Butanol-Water System Using the a-Transglucosidase from Aspergillus niger, Marie-Pierre Bousquet, Rene-Marc Willemot, Pierre F. Monsan, Francois Paul, and Emmanuel Boures. Enzymatic Glycosylation of Aglycones of Pharmacological Significance, Yukio Suzuki and Kei Uchida. Enzymatic Synthesis of Glycosides in Aqueous-Organic Two-Phase Systems and Supersaturated Substrate Solutions, Jeffrey A. Khan, Anna Millqvist-Fureby, and Evgeny N. Vulfson. Use of b-Glucosidase in the Development of Flavor in Wines and Fruit Juices, Yannick Gueguen, Patrick Chemardin, Guilhem Janbon, Alain Arnaud, and Pierre Galzy. Index.

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"...The recommendable book is very useful for students to start experiment work and for scientists and practitioners in doing experimental tasks."-Nahrung "...the individual chapters are well written by subject matter authorities, and provide useful insights to the original research literature. The bench manual format of the book perhaps makes it most useful to active researchers interested in the specific protocols selected for this volume, although a wealth of interesting information is found among the chapter introductions and notes."-ASM News "Each chapter of the volume is armed with necessary literature references, as basic sources of knowledge. The contributions throughout the book are easily readable and understandable, this is why I recommend the book not only to experts in synthetic chemistry, biochemistry, fermentation biotechnology, and applied enzymology, but also to beginners and students who are new to the field of carbohydrate biotechnology.-Models of Chemistry "The protocols are clearly arranged and begin with a short theoretical and commercial background of the method, the list of reagents and equipment and continue with a comprehensive step-by-step description and a set of notes. Such procedure saves time of the user, indicating quickly which problems are likely to arise. The recommendable book is very useful for students to start experimental work and for scientists and practitioners in doing experimental tasks."-Nahrung "...a valuable resource to more-experienced practitioners who require alternative methodologies....this volume will prove a useful resource for researchers of graduate level and above, with an interest in investigating carbohydrate biochemistry as applied to the fields of biotechnology and pharmacology." - Tibtech

EAN: 9781617370748
ISBN: 1617370746
Publisher: Humana Press Inc.
Dimensions: 22.9 x 15.2 x 1.9 centimetres (0.52 kg)
Age Range: 15+ years
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