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Chromatin
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Developments in the analysis of the regulation of chromatin structure have provided much information on their relevance in the control of DNA replication and transcription, making it an important area of research. Chromatin: A Practical Approach provides clear explanations of a wide range of techniques from the initial isolation and fractionation of chromatin and linker histones, to the in vitro assembly of chromatin and nuclear structures in Xenopus egg extracts. Both in vivo and in vitro methods are covered and clear explanations of procedures are provided throughout making this volume an invaluable aid to laboratory workers in biochemistry and molecular biology, especially those whose research encompasses eukaryotic transcription, DNA replication, and chromosome structure.
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Table of Contents

1. Isolation and fractionation of chromatin and linker histones ; 2. Extraction and separation of core histones and non-histone chromosomal proteins ; 3. Mapping chromatin structure in Drosophila ; 4. In vivo footprint and chromatin analysis by LMPCR ; 5. Analysis of nuclear scaffold attachment regions ; 6. Electron microscopy of chromatin ; 7. Counting nucleosome cores on circular DNA using topoisomerase I ; 8. In vitro reconstitution of chromatin and analysis of nucleosome positioning ; 9. Transcriptional and structural analysis of chromatin assembled in vivo ; 10. An assembly of chromatin and nuclear structures in Xenopus egg extracts ; Appendix: List of Suppliers ; Index

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"The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume."--The Quarterly Review of Biology "The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume."--The Quarterly Review of Biology "The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume."--The Quarterly Review of Biology "The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume."--The Quarterly Review of Biology "The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume."--The Quarterly Review of Biology

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