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Circular Dichroism and Linear Dichroism

Increasingly widely used techniques to study molecular structure and interactions, especially of biomacromolecules (proteins and DNA)
-- Only book that explains both techniques from basic principles to complicated theory
-- Enables readers to use and CD and LD in a practical environment.

This book provides an introduction to all those who wish to use the complementary spectroscopic techniques of optical activity (circular dichroism, CD) and optical anisotropy (linear dichroism, LD) for the study of the structure of molecules and interactions between molecules in solution. It emphasizes these techniques and how to use them for both low and high molecular weight molecules. The book begins by describing the principles behind CD and LD and how these techniques can be used in the laboratory without using advanced maths or quantum mechanics. The next chapters describe how both techniques may be applied to the study of biological macromolecules and give a detailed description of how they may be used on small molecules to investigate molecular and electronic structure. The final part contains theoretical derivations of all the equations required for the applications described previously. Specific molecular examples are used to illustrate concepts and to show the reader how to use the techniques in chemical and biological systems. Circular Dichroism and Linear Dichroism is an easy guide to what a prospective user of CD needs to know and explains how LD is not merely an exotic technique only to be practised by experienced spectroscopists, but may be routinely and usefully employed as an aid to molecular structure determination.

Product Details

Table of Contents

1. Spectroscopy, chirality, and oriented systems ; 2. Circular dichroism of biomolecules ; 3. Linear dichroism of biomolecules ; 4. Linear dichroism of small molecules ; 5. Analysis of circular dichroism: electric dipole allowed transitions ; 6. Analysis of circular dichroism: magnetic dipole allowed transitions and magnetic CD ; 7. Circular dichroism formalism ; A1. Vectors ; A2. Determination of equilibrium binding constants ; A3. Momentum-dipole equivalence


'The book is most likely to be of value to research workers with access to appropriate equipment.' Aslib Book Guide, Vol. 62, No. 7, July 1997

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