Part I. Introduction. Developmental Biology Protocols: Overview II, Rocky S. Tuan and Cecilia W. Lo. Part II. Organogenesis. Drosophila as a Genetic Tool to Define Vertebrate Pathway Players, Nancy M. Bonini. Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis, Makoto Asashima, Takashi Ariizumi, Shuji Takahashi, and George M. Malacinski. Analysis of Mammary Gland Morphogenesis, Calvin D. Roskelley, Colleen Wu, and Aruna M. Somasiri. Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro, H. Joseph Yost. Craniofacial Development and Patterning, Harold Slavkin, Glen Nuckolls, and Lillian Shum. Craniofacial Skeletel Morphogenesis In Vitro, Roy C. Ogle. Skeletal Morphogenesis, Stefan Mundlos. Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration, David L. Stocum. Retroviral Infection of T-Cell Precursors in Thymic Organ Culture, Lisa M. Spain, Lisa L. Lau, and Yousuke Takahama. Assay for the Isolation of Hepatogenic Factors: Key Molecules in Hepatocyte Formation, Johannes A. A. Spijkers, Theodorus B. M. Hakvoort, and Wouter H. Lamers. Skin Morphogenesis: Embryonic Chicken Skin Explant Cultures, Cheng-Ming Chuong. Apoptosis in Development, Lin Lin and Zahra F. Zakeri. Methods to Detect Patterns of Cell Death in Drosophila, Nancy M. Bonini. Part III. Abnormal Development and Teratology. Mechanisms of Neurulation, Gary C. Schoenwolf and Jodi L. Smith. Neurulation and Neural Tube Closure Defects, Andrew Copp, Patricia Cogram, Angeleen Fleming, Dianne Gerrelli, Deborah Henderson, Andrew Hynes, Maria Kolatsi-Joannou, Jennifer Murdoch, and Patricia Ybot-Gonzales. Neural Tube Defects, Takayuki Inagaki, Jodi L. Smith, Marion L. Walker, and Gary C. Schoenwolf. Experimental Manipulation and Morphometric Analysis of Neural Tube Development, Mary E. Desmond and Patricia A. Haas. Isolation of Neuroepithelium and Formation of Minispheres, Mary E. Desmond and Marcia C. Field. Examination of Normal and Abnormal Placentation in the Mouse, Michael R. Blackburn. Palatal Dysmorphogenesis: Palate Organ Culture, Barbara D. Abbott. Palatal Dysmorphogenesis: Quantitative RT-PCR, Gary A. Held and Barbara D. Abbott. Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities, Sheila M. Bell, Claire M. Schreiner, and William J. Scott. Assessment of Laterality Defects in Rodent Embryos, Masahiko Fujinaga. Cardiac Morphogenesis and Dysmorphogenesis: I. Normal Development, Andy Wessels and Roger Markwald. Analysis of Two Aspects of Left-Right Patterning of the Vertebrate Heart: Heart Tube Position and Heart Tube Chirality, Alvin J. Chin. Biologically Based Risk Assessment Models for Developmental Toxicity, Christopher Lau and R. Woodrow Setzer. Part IV. Screening and Mapping of Novel Genes and Mutations. Positional Cloning, Lin Xu and Yefu Li. Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmentally Regulated Genes in the Mouse, Gary E. Lyons, Bradley J. Swanson, Melissa A. Haendel, and Joshua Daniels. PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes, Jian Zhang and Mary Lou King. Analysis of mRNA Expression During Preimplantation Development, Keith E. Latham, Elena De La Casa, and Richard M. Schultz. Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Embryos, Akihiko Shimono and Richard R. Behringer. HPLC-Based mRNA Differential Display, Thomas B. Knudsen. Part V. Transgenesis: Production and Gene Knockout. Production of Transgenic Drosophila, Miki Fujioka, James B. Jaynes, Amy Bejsovec, and Michael Weir. Transgenic Manipulation of the Sea Urchin Embryo, Jonathan P. Rast. Transgenic Zebrafish, Shuo Lin. Production of Avian Chimeras and Germline Transmission, Kristin L. Woods, Scott Schau, Mary Ellen Clark, Jacqueline A. Bonselaar, and Robert J. Etches. Incorporation of Genetically Modified Cells into Chicken Chimeras, Laura D.
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"We live in a time of rising expectations. Being a developmental biologist at the turn of the millenium demands expertise in embryological manipulation, viral gene expression, in situ hybridization, videomicroscopy, in vitro assays, transgenic embryo production, cell lineage analysis, PCR techniques, and computer-enhanced imaging technology. Rocky Tuan and Cecilia Lo have done the field (and each scientist within it) a wonderful service by collecting and editing the protocols of the masters of each echnique. More than 100 papers spanning these areas (and more) patiently lead one through each method, giving details on what brand of microscope slides to use, what centrifuge tubes work best, and where to purchase each piece of equipment. Copious notes provide details based on the laboratories' experiences of what works and what doesn't. Developmental Biology Protocols is a library in itself and will be essential for every laboratory of developmental biology. This is the collection to get before your next grant application is due."-Scott Gilbert, Department of Biology, Swarthmore College, Swarthmore, PA "The range of techniques, from viral vectors to imaging, and of organisms, from sea urchins to mammals, is comprehensive yet focused. This work should be an invaluable aid to those interested in all aspects of comparative development. This work is logically organized and presents a great opportunity for not only picking up a technique but also placing it in a logical context. Well done!" -Barbara B. Knowles, Director of Research, Jackson Labs, Bar Harbor, ME "Among similar laboratory manuals Developmental Biology Protocols edited by Tuan and Lo impress by their breadth of coverage, timeliness, and scrupulous attention to detail. . .these protocols will be an invaluable aid to every geneticist and developmental biologist."-Davor Solter, Director of Developmental Biology, Max-Planck Institute, Freiburg, Germany