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Electrophoresis in Practice
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Table of Contents

PART I: FUNDAMENTALS Introduction Electrophoresis Isotachophoresis Isoelectric Focusing Blotting Interpretation of Electropherograms Proteome Analysis Instrumentation PART II: EQUIPMENT AND METHODS Equipment for Part II Method 1: PAGE of Dyes Method 2: Agarose and Immuno Electrophoresis Method 3: Titration Curve Analysis Method 4: Native PAGE in Amphoteric Buffers Method 5: Agarose IEF Method 6: PAGIEF in Rehydrated Gels Method 7: Horizontal SDS-PAGE Method 8: Vertical PAGE Method 9: Semi-dry Blotting of Proteins Method 10: IEF in Immobilized pH Gradients Method 11: High-resolution 2D Electrophoresis Method 12: PAGE of Double Stranded DNA Method 13: Native PAGE of Single Stranded DNA Method 14: Denaturing Gradient Gel Electrophoresis Method 15: Denaturing PAGE of DNA Appendix A: Trouble-shooting References Index

About the Author

Born in 1951, Reiner Westermeier gained his diploma in engineering in 1976, and his doctorate in 1981 at the Technical University of Munich, Germany. He remained at the TU engaged in the development of new electrophoresis methods and applications until 1983, a period that also involved several international collaborations and lecturing tours. From 1984 to 1987 Dr. Westermeier was employed as an electrophoresis products and applications specialist at LKB Instrument GmbH thereafter until 1990 at Pharmacia Biotech. He founded ETC Elektrophorese-Technik in 1991 for the development of new electrophoresis methods, media, and equipment. From January 1996 to July 1997 he was manager of scientific support Europe at Pharmacia Biotech in Freiburg, Germany. He has been at Amersham Pharmacia Biotech since July 1997, and its Senior Scientist for Proteomics since January 2002. Reiner Westermeier is the author of several publications and two books, both published by WILEY-VCH.

Reviews

From the reviews of previous editions: "The illustrations are informative and to the point...The book clearly illustrates the competence of the author. He generously shares his experience and knowledge with the reader, especially in the field of gel-based electrophoresis...an excellent reference to help the reader to become acquainted with the field of electrophoresis. The book contains theoretical and ample practical information that serves not only the practician in the electrophoresis laboratory, but also students and lecturers in electrophoretic methods." (Clinical Chemistry) "The rigorous description of each method along with the extensive figures will easily allow the novice to reproduce these methods in the laboratory" (The Analyst) "An excellent book which we recommend greatly and which has not to be missed in any laboratory of cellular and molecular biology which respects itself." (Cellular and Molecular Biology) "As a comprehensive guide to the huge variety of electrophoretic methods now available, this is very good value. The superb troubleshooting appendix almost justifies the price on its own." (Laboratory Equipment Digest)

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