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Enzyme Engineering

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Table of Contents

Keynote Papers.- Genetic Engineering.- The Photosynthetic Apparatus.- Session I. Production and Extraction of Microbial Enzymes.- Increasing the Production of Enzymes Via Fermentation.- Cultural Conditions for the Microbial Production of ?-Tyrosinase and Tryptophanase.- Batch Culture Experiments in the Optimization of Continuous Fermentation of an Intracellular Enzyme.- Properties of Heat Stable Enzymes of Extreme Thermophiles.- Aqueous Two-Phase Systems for the Large-Scale Purification of Enzymes.- Contributed Papers: Procedure for the Simultaneous Large-Scale Isolation of Pullulanase and 1,4-?-Glucan Phosphorylase Involving Liquid-Liquid Separations.- Purification and Immobilization of a Fungal ?-Galactosidase (Lactase).- A Xylanase from Schizophyllum commune.- Session II. Industrial Applications of Enzymes: Engineering Aspects.- Immobilized Lactase for Whey Hydrolysis: Stability and Operating Strategy.- Reaction Engineering Aspects of Continuous Operation with Biocatalysts.- Economical Aspects of Continuous Operation with Biocatalysis.- Contributed Papers: Pressure Drop Across Compressible Beds.- Heterogeneous Kinetics of Two-Substrate Enzymic Reactions Under Diffusional Limitations.- Membrane Reactor-Separator for Continuous Enzymatic Regeneration of ATP.- Immobilized Glucose-Oxidase/Catalase: Deactivation in a Differential Reactor.- Effect of Design Parameter on the Performance of Immobilized Glucose Isomerase Reactor System.- Immobilization of Acetate Kinase on Functionalized Solid-Core Polymeric Beads.- Session III. Study of Modified, Stabilized, and Immobilized Enzymes.- Surface Modification of Proteins.- Staphylococcal Nuclease Immobilized on Agarose Gel.- Enzyme Collagen Membranes in Reactors and for Analytical Purposes.- Contributed Papers: Analysis of the Reaction Kinetics of Single Esterase-Sepharose Beads by Microfluorometry.- A New Kinetic Method for Analyzing the Parameters of Immobilized Aldolase.- Interaction of Enzymes with Synthetic Polymers.- Proteolysis of Immobilized Lactate Dehydronase in the Presence of Pyruvate and NADH.- Synthesis and Kinetic Properties of a New NAD+ Derivative Carrying a Vinyl Group.- Isocyanide Derivatives of Polysaccharides as Supports for Enzyme Immobilization.- Enzymes on Magnetic Supports.- Oxirane-Acrylic Beads, Preparation 2878-C.- Immobilization of 3?-Hydroxysteroid Dehydrogenase Isolated from Streptomyces griseocarneus.- A Method to Prepare Bead-Shaped Immobilized Enzyme.- Immobilization of Hydrogenase from Alcaligenes eutrophus H 16 on Glass Beads.- Hemisynthesis of Aminoglycosides.- Session IV. New Medical Applications for Enzymes.- Enzyme-Polyethylene Glycol Adducts: Modified Enzymes with Unique Properties.- Carrier-Bound Detoxifying Enzymes for an Extracorporeal Detoxification of Endogenous and Exogenous Toxins.- Biomedical Applications of Enzymatic Fibres.- Liposomes as Carriers of Proteins: Possible Medical Applications.- Galactose Conversion Using a Microcapsule Immobilized Multienzyme Cofactor Recycling System.- Hollow Fiber-Entrapped Liver Microsomes: A Potential Extracorporeal Drug Detoxifier.- Contributed Papers: Enzymatic Conversion of Cyclohexane to Cyclohexanol by Isolated Rat Liver Microsomes (MS).- Hydrolysis of Glucuronides with Immobilized Enzymes.- Immobilization of Neuraminidase for the Treatment of Tumor Cells.- Enzyme Thermistor Analysis in Clinical Chemistry and Environmental and Process Control.- Nylon Tube Bound Enzymes in Systems for the Determination of Substrates in Serum and Urine by Electrochemical and Calorimetric Detectors.- Analytical Application of Collagen Enzymatic Membranes for a Glucose Sensor.- Immobilization of Enzyme on PMG and its Application to the Urea Monitoring Apparatus.- Session V. Industrial Applications for Enzymes: State of the Art.- Industrial Applications of Immobilized Enzymes: State of the Art.- Regeneration of ATP by Immobilized Microbial Cells and its Utilization for the Synthesis of Nucleotides.- Production of 6APA in the Penicillin G Fermentation Plant by Using Fiber-Entrapped Penicillin Amidase.- Enzymatic Deacetylation of Cephalosporins.- Enzymatic Production of Sugars from Hemicellulose.- Enzymes and Separation Processes.- Enzymatic Hydrolysis and Protein Ultrafiltration for Converting Acid Whey to Valuable Products.- Contributed Papers: Model Proteolysis of ?-Casein with Immobilized Trypsin and its Technological Significance.- Detoxification of Organophosphate Pesticides Using Immobilized Enzymes.- Production of L-Tryptophan.- Electrochemical Assay of Oxidoreductase Enzymes.- Design Considerations and Operational Strategies of a Commercial Glucose Isomerase Reactor System.- Session VI. Multienzyme Systems, Immobilized Whole Cells, and Organelles.- Enzyme Sequences in the Living Cell.- Immobilization of Enzymes, Microbial Cells, and Organelles by Inclusion with PhotoCrosslinkable Resins.- Immobilized Cell Systems.- Steroid Conversion Using Immobilized hiving Microorganisms.- Microbial Cells Immobilized and Living on Solid Supports and Their Application to Fermentation Processes.- Microbial Electrode: BOD Sensor.- Contributed Papers: New Method for Immobilization of Microbial Cells and its Industrial Application.- Polymer Entrapment of Microbial Cells: Preparation and Reactivity of Catalytic Systems.- Benzene Metabolism by Bacterial Cells Immobilized in Polyacrylamide Gel.- Cellulose Bead Entrapped Whole Cell Glucose Isomerase in Fructose Syrup Production.- Properties of a Cell-Bound Glucose Isomerase.- Session VII. Fundamentals of Solid Phase Biochemistry.- Hydrophobic Interactions in Purification and Utilization of Enzymes.- Relative Importance of Diffusion Layer Resistance and Microenvironmental Effects on the Effectiveness of Immobilized Enzyme Reactors.- Studies of Hemoglobin and Allosteric Enzymes Under Artificial Conformational Constraints.- Determination of Michaelis Menten Parameters for Immobilized Enzymes: Detection and Measurement of Diffusion.- Studies with Immobilized Toxins.- Contributed Papers: Immobilized Protein Modification Reagents.- On the Mechanism of Protein Cross-Linking with Glutaraldehyde.- Synthesis of Water Soluble Polymers with Covalently Bound General Ligands.- Soluble NAD+-Derivatives of High Molecular Weight in Enzymic Recycling Systems.- The Use of Enzymes for Design of Light Sensitive Silverless Materials: Photoenzography.- Continuous Measurements on Immobilized Cells by a Mass Filter.- Enzyme Activity at a Gas Solid Interphase: Oxidation of Methanol to Formaldehyde.- Some Reactive Carriers and Immobilized Enzymes.- Immobilization of Proteins on Polyhydrazides.- Studies of Insoluble Organic/Inorganic Composits of Glucose Isomerase.- Session VIII. Application of Affinity Methods and New Purification Procedures.- Pressure-driven Affinity Sorption of Trypsin Inhibitors.- Measurement of ATP and Ligand-ATP Conjugates by Enzymic Cycling with Co-immobilized Hexokinase and Pyruvate Kinase.- Affinity Therapy.- Contributed Papers: Principles of Multi-Enzyme Purification by Affinity Chromatography.- Affinity Chromatography of Proteolytic Enzymes.- Guanosine Nucleotide Analogues as General Ligands in Affinity Chromatography.- Physical-Chemical Factors Influencing the Resolution in Affinity Chromatography.- Use of Affinity Chromatography for Determination of Dissociation Constants of Complexes of Trypsin and Chymotrypsin with their Free and Immobilized Inhibitors.- Purification of Pyruvate Dehydrogenase by Affinity Chromatography.- Studies on the Purification of Aspergillus niger Endopolygalacturonase by Agarose Gel Chromatography.- Affinity Chromatography: Recent Advances.- Guidelines for the Characterization of Immobilized Enzymes: A Proposal.- List of Participants.

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