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Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.
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Table of Contents

Section I: B-cell Epitope Mapping 1. What Is a B Cell Epitope? Marc H. V. Van Regenmortel Subsection A: Whole Antigen Methods 2. Structural Basis of Antibody-Antigen Interactions Eric J. Sundberg 3. Epitope Mapping of Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy Osnat Rosen and Jacob Anglister 4. A Solid-Phase Mutual Inhibition Assay with Labeled Antigen Masahide Kuroki 5. Epitope Mapping by Surface Plasmon Resonance Par Safsten 6. Proteolytic Fragmentation for Epitope Mapping Maria R. Mazzoni, Francesca Porchia, and Heidi E. Hamm 7. Epitope Mapping by Proteolysis of Antigen-Antibody Complexes Suraj Dhungana, Jason G. Williams, Michael B. Fessler, and Kenneth B. Tomer 8. Identifying Residues in Antigenic Determinants by Chemical Modification Howard M. Reisner and Roger L. Lundblad 9. Epitope Mapping by Differential Chemical Modification of Antigens Suraj Dhungana, Michael B. Fessler, and Kenneth B. Tomer Subsection B: Peptide Library Approaches 10. Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides Michael W. Heuzenroeder, Mary D. Barton, Thiru Vanniasinkam, and Tongted Phumoonna 11. Antibody Epitope Mapping Using SPOTTM Peptide Arrays Ulrich Reineke and Robert Sabat 12. Peptide Microarrays for Profiling of Modification State Specific Antibodies Johannes Zerweck, Antonia Masch, and Mike Schutkowski 13. Epitope Mapping Using Phage Display Peptide Libraries Volker Boettger and Angelika Boettger 14. Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries Ulrich Reineke 15. Antibody Specificity Profiling on Functional Protein Microarrays Dawn R.Mattoon and Barry Schweitzer 16. Peptide Microarrays for Determination of Cross-Reactivity Alexandra Thiele Subsection C: Profiling of Antibody Signatures in Biological Fluids 17. Epitope Mapping Using Randomly Generated Peptide Libraries Juliane Bongartz, Nicole Bruni, and Michal Or-Guil 18. Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients With Autoimmune Disease Peter Lorenz, Michael Kreutzer, Johannes Zerweck, Mike Schutkowski, and Hans-Jurgen Thiesen 19. Microarrayed Allergen Molecules for Diagnostics of Allergy Jing Lin, Ludmilla Bardina, and Wayne G. Shreffler 20. Humoral Response to Immunoselected Phage-Displayed Peptides by Microarray-Based Method Lina Cekaite, Eiving Hovig, and Mouldy Sioud Subsection D: Antigen Expressed from Recombinant DNA 21. Epitope Mapping Using Homolog-Scanning Mutagenesis Lin-Fa Wang 22. Epitope Mapping by Region-Specified PCR-Mutagenesis Tsutomu Mikawa, Masayuki lkeda, and Takehiko Shibata 23. Epitope Mapping Using Phage-Display Random Fragment Libraries Lin-Fa Wang and Meng Yu Subsection E: B-Cell Epitope Prediction 24. Prediction of Linear B-cell Epitopes Ulf Reimer Section II: T-Cell Epitope Mapping 25. Molecular Recognition of Diverse Ligands by T Cell Receptors Eric J. Sundberg 26. Identification of Human MHC Class I Binding Peptides using the iTOPIATM Epitope Discovery System Markus Wulf, Petra Hoehn, and Peter Trinder 27. T-Cell Epitope Mapping in Mycobacterium Tuberculosis Using PepMixes Created by Micro-Scale SPOTTM Synthesis Marisa Frieder and David M. Lewinsohn 28. High-Throughput T Cell Epitope Discovery Through MHC Peptide Exchange Sine Reker Hadrup, Mireille Toebes,


From the reviews of the second edition: "This is a beautifully presented and very well-written book on the subject ! . The diagrams and data are of a very high standard and black and white ! . a fantastic lab resource and would benefit any immunology lab where epitope mapping is being or will be used. ! a useful reference book of interest to staff from Pls to PhD students, providing both a thorough background and working methods. ! a high quality and reader friendly book and I would highly recommend it." (Barbara Guinn, Immunology News, August, 2009)

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