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In recent years, developmental biology has undergone a revolution. The introduction of new techniques of molecular and cell biology, couples with those designed by classical embryologists, has provided a new approach to the study of mechanisms that control early animal development. As new techniques continue to be developed and refined, there is now an immediate need for a book that brings together approaches from many different disciplines as they apply to embryos. This volume brings together easy-to-follow protocols and practical instructions for all of the main techniques in classical embryo manipulation, from traditional embryology to cellular and molecular methods. It includes reprints of all the stage tables in common use for the main laboratory species. It continues with practical instruction on techniques for ebryological manipulations such as fate mapping, cell lineage analysis, ablation and transplantation, together with molecular methods for constructing cDNA libraries, cloning genes, analyzing their expression and disrupting their action during development. Each chapter is written by authorities in the field. An addition to the book are reprints of the most commonly-used staging tables for zebrafish, amphibian and chick embryos. This book should provide a guide for those who wish to broaden their approach to cover different animal species, or to move across the boundaries between molecular, cellular and embryological strategies.
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Table of Contents

PART I: BASIC TECHNIQUES: OBTAINING AND HANDLING EMBRYOS ; 1. Drosophila embryos ; 2. Nematode embryos ; 3. Embryos and larvae of invertebrate deuterostomes ; 4. Zebrafish embryos ; 5. Xenopus ; 6. Avian embryos ; 7. Post-implantation mammalian embryos ; 8. Simple tips for photomicrography or embryos ; PART II: EXPERIMENTAL EMBRYOLOGY ; 9. Fate maps and cell lineage analysis ; 10. Microinjection into Drosophila embryos ; 11. Cell transplantation in Xenopus ; 12. Transplantation in avian embryos ; 13. Chick limb buds ; 14. Nuclear transplantation ; 15. Laser ablation of cells ; PART III: CELLULAR TECHNIQUES ; 16. Studying cell movements in vivo by time-lapse video microscopy ; 17. Induction assays in Xenopus ; 18. Culture of avian neural crest cells ; 19. ES cells and gene targeting ; 20. Introduction of genes using retroviral vectors ; PART IV: MOLECULAR TECHNIQUES ; 21. Immunocytochemistry of embryonic material ; 22. RNase protection assays ; 23. cDNA library construction ; 24. Analysis of gene expression by RT-PCR ; 25. Cloning genes using PCR ; 26. In situ hybridisation ; Appendices ; I: Stage tables ; a. Westerfield for zebrafish embryos ; b. Nieukoop & Faber for Xenopus laevis ; c. Eyal-Giladi & Kochav for early chick ; d. Hamburger & Hamilton for later chick ; II: Suppliers of specialist items ; Index


'this book will be widely welcomed ... the ability to tackle a developmental problem in a variety of different species is increasingly valuable; having the relevant methods to hand will make this type of approach easier and more productive ... highly recommended and sensibly priced for the biologist on a shoestring.' Justin Grindley, TIG, September 1994, Vol. 10. No. 9 'This book will be very useful as a guide to organize university practicals or a full practical course ... just reading the book gives a good glimpse of how is the real thing ... books like this one are very welcome.' Juan Pablo Couso, University of Cambridge, BioEssays, Vol. 16, No. 2 - February 1993 'a very useful reference for either a newcomer to developmental biology research or an experienced developmental biologist who would like to look at the use of a different developmental or experimental system' Michael Fietz, Department of Zooology, Oxford, BSDB Newsletter 29 'The volume will be an essential laboratory manual for all developmental biologists.' Aslib Book Guide, Vol. 59, No. 3, March 1994 'Developmental biology is entering an exciting new age. More and more, developmental biologists choose interdisciplinary and interorganismal approaches to study the particular problems they are interested in,. This book is directed towards this kind of new-age developmental biologist. The scope of the book is truly broad. Techniques for growing embryos from flies, worms, zebrafish, Xenopus, rats and an array of marine invertebrates are covered. The articles are all written by experts in the field ... Each of the individual contributions is extremely clear and to the point. One of the most useful features of the book is the appendix ... This book nicely fills that gap between theory and practice. The revolutionary thing about this book is the inclusion of discussion of flies and worms ... a true give-and-take of information between all animals is on the horizon.' Kathryn V. Anderson, University of California, Berkeley, Trends in Cell Biology Vol. 4, March 1994

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