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Finfish and Shellfish Bacteriology Manual

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Significant advances in the field of the microflora of aquatic animals, including fish and invertebrates, necessitate a practical laboratory text providing the current methods available for the bacteriological study of health and diseased fish and shellfish. Practical rather than theoretical, the manual will be useful for individuals working in aquatic, environmental, and veterinary science, and in aquaculture, public health, and aquatic research.Methods are presented for the isolation and identification of bacterial pathogens in fish and shellfish and zoonotic pathogens in shellfish. The step-by-step presentation of procedures guides students in aquatic health and newcomers to the diagnostic laboratory while providing a refresher course for the more experienced laboratory employee. Numerous tables and illustrations further assist readers. Thorough coverage of basic bacteriological techniques ensures that even the inexperienced aquatic health diagnostician lacking a background in microbiology will need no additional resources. An accompanying CD-ROM provides color images of relevant bacteria in relevant media, whole plate and close up, to aid in laboratory identification.The effective, easy-to-follow organization and wealth of valuable information in the Finfish and Shellfish Bacteriology Manual will make it the primary sourcebook in any laboratory. Whether it's used as an occasional quick reference by the experienced fish diagnostician or as a tool to build diagnostic capabilities from the ground up, this book rises to the challenge.
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Table of Contents

Preface. Introduction. 1. Finfish Bacteriology. 2. Sample Collection to Detect Bacterial Infection in Finfish. 3. Packaging Fish for Transport. 4. Aseptic Bacterial Examination of Finfish. 5. Shellfish Bacteriology. 6. Sample Collection to Detect Bacterial Infection in Shellfish. 7. Sampling and Packaging Bivalves and Crustaceans for Transport. 8. Aseptic Bacterial Examination of Bivalves. 9. Aseptic Bacterial Examination of Lobsters. 10. Diagnostic Methodology in Finfish and Shellfish Bacteriology. 11. Interpretation of Diagnostic Tests. 12. Bacteriology Culture Media. 13. Pure-Culture Techniques. 14. Staining Methods. 15. Colony Identification. 16. Biochemical Activities of Bacteria. 17. Serology. 18. Antibiotic Testing. 19. Specific Techniques Used for Finfish Bacteriology. 20. Specific Techniques Used for Shellfish Bacteriology. 21. Enumeration of Bacteria by the Plate Count Technique. 22. Public Health Indicator Oganisms in Bivalves. Appendix. Bibliography.

About the Author

Kimberley A. Whitman BSc., specializes in microbiology, and has 20 years experience working as a fish health bacteriologist/biologist with government agencies, academic institutions, and industry. Currently, Ms. Whitman is working with Malaspina University College, Nanaimo, British Columbia as a fish health instructor. She also is employed with the private sector implementing a quality assurance program for a marine extraction plant, and is the owner of OHB Enterprises as a consultant for international aquatic facilities.


"Although, at first sight, the title of this book may suggest that it has no relevance to parasitologists studying eukaryotic pathogens, closer examination reveals it to have many features that commend it as a valuable asset in the parasitology laboratory, particularly for those with an interest in fish and shellfish." --Parasitology, 2004

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