Table of Contents

Basics of Fluorescence. Basics of Microscopy. Basics of FLIM Microscopy. Probes for FLIM Microscopy. Dynamic FLIM Microscopy Imaging Using Gating Camera (Time Domain). Video Rate FLIM Imaging Microscopy (Frequency Domain). Laser Scanning Confocal FLIM Microscopy. Multiphoton-Excitation Time-Correlated Single-Photon Counting FLIM Microscopy. SLIM for Multispectral Imaging Microscopy. Nonlinear Curve-Fitting Methods for FLIM Data Analysis. Global FLIM Data Analysis. New Approaches in FLIM Data Analysis: Polar Plot. Critical Review of FLIM Applications.

About the Author

Ammasi Periasamy, University of Virginia, Charlottesville, USA

Robert M. Clegg, University of Illinois, Urbana-Champaign, USA

Reviews

"…this book represents a clear presentation of the theory, instrumentation, and data analysis of FLI microscopy and is enhanced by the critical discussions of assumptions, limitations and confounding elements… ."
—Barry R. Masters, MIT, Microscopy and Microanalysis"…an excellent and comprehensive treatment of FRET in biology investigation. It has a good balance of basic theory and cutting-edge development in both techniques and applications."
—Peter So, Ph.D., MIT"An amazingly comprehensive reference—I can’t imagine anyone looking for information there and not finding what they need. It is quite essential for any lab doing FLIM."
—Guy Cox, University of Sydney, Australia

"…this book represents a clear presentation of the theory, instrumentation, and data analysis of FLI microscopy and is enhanced by the critical discussions of assumptions, limitations and confounding elements… ."
—Barry R. Masters, MIT, Microscopy and Microanalysis"…an excellent and comprehensive treatment of FRET in biology investigation. It has a good balance of basic theory and cutting-edge development in both techniques and applications."
—Peter So, Ph.D., MIT"An amazingly comprehensive reference—I can’t imagine anyone looking for information there and not finding what they need. It is quite essential for any lab doing FLIM."
—Guy Cox, University of Sydney, Australia