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Genome analysis has undergone a period of explosive growth in recent years. This book describes up-to-date methods for genome mapping, arranged in order of increasing resolution, from linkage analysis to restriction mapping. Topics covered include linkage mapping of human, animal, and plant genomes; mapping of quantitative trait loci; radiation hybrid mapping; HAPPY mapping; somatic cell hybrids; flow-sorted and microdissected chromosomes; fluorescence in situ hybridization; contig assembly; chromosome walking; and long-range restriction mapping. Two appendices describe sources of freely available biological and computational resources relevant to genome analysis.
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Table of Contents

1. Human linkage mapping ; 2. Linkage mapping of plant and animal genomes ; 3. Linkage mapping of quantitative trait loci in plants and animals ; 4. Radiation hybrid mapping ; 5. HAPPY mapping ; 6. Construction and use of somatic cell hybrids ; 7. The use of flow-sorted chromosomes in genome mapping ; 8. The use of microdissected chromosomes in genome mapping ; 9. Fluorescence in situ hybridization ; 10. Contig assembly by fingerprinting ; 11. Chromosome wallking ; 12. Long-range restriction mapping of genomic DNA ; Appendix 1 Core list of suppliers ; Appendix 2 Non-commercial source for genome mapping ; Appendix 3 Bioinformatics for genome mapping

About the Author

Dr Paul Dear, MRC Centre, Hills Road, Cambridge CB2 2QH. Email: phd@mrc-lmb.cam.ac.uk. Tel: 01223 402190. Fax: 01223 412178

Reviews

"This book is part of a large series of instruction manuals on diverse topics in biochemistry and molecular biology. The 12 individually authored chapters in this volume contain practices and protocols of interest to the advanced researcher in the field of genome mapping. Each chapter contains an introduction, lists of materials, references, and several protocols. The text is indexed and illustrated with tables and figures, some of which are in color. A list of abbreviations is provided." -- Biosis, Vol 50, Issue 9, Sept 22, 1998 "This book is part of a large series of instruction manuals on diverse topics in biochemistry and molecular biology. The 12 individually authored chapters in this volume contain practices and protocols of interest to the advanced researcher in the field of genome mapping. Each chapter contains an introduction, lists of materials, references, and several protocols. The text is indexed and illustrated with tables and figures, some of which are in color. A list of abbreviations is provided." -- Biosis, Vol 50, Issue 9, Sept 22, 1998 "This book is part of a large series of instruction manuals on diverse topics in biochemistry and molecular biology. The 12 individually authored chapters in this volume contain practices and protocols of interest to the advanced researcher in the field of genome mapping. Each chapter contains an introduction, lists of materials, references, and several protocols. The text is indexed and illustrated with tables and figures, some of which are in color. A list of abbreviations is provided." -- Biosis, Vol 50, Issue 9, Sept 22, 1998 "This book is part of a large series of instruction manuals on diverse topics in biochemistry and molecular biology. The 12 individually authored chapters in this volume contain practices and protocols of interest to the advanced researcher in the field of genome mapping. Each chapter contains anintroduction, lists of materials, references, and several protocols. The text is indexed and illustrated with tables and figures, some of which are in color. A list of abbreviations is provided." -- Biosis, Vol 50, Issue 9, Sept 22, 1998

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