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Handbook of Phycological Methods: Culture Methods and Growth Measurements


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Contributors; Editor's preface; Introduction Janet R. Stein; Part I. Isolation and Purification: 1. Growth media - freshwater H. Wayne Nichols; 2. Growth media - marine J. McLachlan; 3. Methods for microscopic algae Robert W. Hoshaw and James R. Rosowski; 4. Methods for microflagellates and nannoplankton Robert R. L. Guillard; 5. Methods for macroscopic algae A. R. O. Chapman; 6. Methods for coenocytic algae Joanna Ziegler Page; 7. Methods for Cyanophyceae Mary Mennes Allen; 8. Special methods - micromanipulators Jahn Throndsen; 9. Special methods - virus detection in Cyanophyceae Robert S. Safferman; 10. Special methods - dry soil samples Richard C. Starr; Part II. General Equipment and Methods: 11. Apparatus and maintenance Richard C. Starr; 12. Sterilization R. D. Hamilton; 13. Preservation by freezing and freeze-drying Osmunf Holm-Hansen; 14. Toxic and inhibitory materials associated with culturing William F. Blankley; Part III. Special Culture Methods: 15. Continuous culture - a method for the production of unicelluar algal foods Ravenna Ukeles; 16. Mass culture Glen Hemerick; 17. Light-temperature gradient plate Chase Van Baalen and Peter Edwards; 18. Growth with organic carbon and energy sources Alasdair H. Neilson, William F. Blankley and Ralph A. Lewin; Part IV. Growth Measurements: 19. Division rates Robert R. L. Guillard; 20. Growth measurements by analysis of carbon David W. Menzel and William M. Dunstan; 21. Dry weight, packed cell volume and optical density Constantine Sorokin; 22. Coulter counter for phytoplankton T. R. Parsons; 23. Pigment analysis Eugene Hansmann; 24. Intracellular growth rates Paul B. Green; Part V. Bioassay: 25. Bioassay: biotin A. F. Carlucci; 26. Bioassay: cyanocobalamin A. F. Carlucci; 27. Bioassay: thiamine Kenneth Gold; 28. Bioassay: vitamin content of algae K. V. Natarajan; Part VI. Index.

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This 1973 book was the first comprehensive collection of techniques for culturing algae.

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