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1. General Review on In Vitro Hepatocyte Models and their Applications Christiane Guguen-Guillouzo and Andre Guillouzo 2. Human Foetal Hepatocytes: Isolation, Characterization, and Transplantation Anne Weber, Thomas Touboul, Sylvie Mainot, and Dominique Mahieu-Caputo 3. Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue Edward L. LeCluyse and Eliane Alexandre 4. Optimization of the Cryopreservation of Primary Hepatocytes Nicola J. Hewitt 5. Cryopreservation of Human Hepatocytes for Clinical Use Ragai R. Mitry, Sharon C. Lehec, and Robin D. Hughes 6. Hepatocyte Differentiation Katy M. Olsavsky Goyak, Elizabeth M. Laurenzana, and Curtis J. Omiecinski 7. Reversible Manipulation of Apoptosis Sensitivity in Cultured Hepatocytes by Matrix Mediated Manipulation of Signaling Activities Patricio Godoy, Markus Schug, Alexander Bauer, and Jan G. Hengstler 8. Markers and Signaling Factors for Stem Cell Differentiation to Hepatocytes: Lessons from Developmental Studies Frederic Lemaigre 9. Hepatic Stem Cells Robert E. Schwartz and Catherine Verfaillie 10. Hepatic Stem Cells and Liver Development Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, and Naoya Kobayashi 11. Generation of Hepatocytes from Human Embryonic Stem Cells Neta Lavon 12. Isolation and Culture of Adult Human Liver Progenitor Cells: In vitro Differentiation to Hepatocyte-Like Cells Sabine Gerbal-Chaloin, Cedric Duret, Edith Raulet, Francis Navarro, Pierre Blanc, Jeanne Ramos, Patrick Maurel, and Martine Daujat-Chavanieu 13. The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies Marie-Jeanne Marion, Olivier Hantz, and DavidDurantel 14. Transdifferentiation of Pancreatic Cells to Hepatocytes Chia-Ning Shen and David Tosh 15. Evaluation of Drug Metabolism, Drug-Drug Interactions, and In vitro Hepatotoxicity with Cryopreserved Human Hepatocytes Albert P. Li 16. The Use of Human Hepatocytes to Investigate Drug Metabolism and CYP Enzyme Induction Sylvie Klieber, Francois Torreilles, Francois Guillou, and Gerard Fabre 17. The Use of Hepatocytes to Investigate UDP-Glucuronosyltransferases and Sulfotransferases Sylvie Fournel-Gigleux, Michael W.H. Coughtrie, Mohamed Ouzzine, and Jacques Magdalou 18. The Use of Hepatocytes to Investigate Drug Uptake Transporters Kazuya Maeda and Yuichi Sugiyama 19. Metabonomic Studies on Human Hepatocyte in Primary Culture Vincent Croixmarie, Thierry Umbdenstock, Olivier Cloarec, Amelie Moreau, Jean-Marc Pascussi, Yannick Parmentier, Claire Boursier-Neyret, and Bernard Walther 20. The Application of HepRG Cells in Evaluation of Cytochrome P450 Induction Properties of Drug Compounds Tommy B. Andersson 21. The Use of Hepatocytes to Investigate Drug Toxicity Maria Jose Gomez-Lechon, Jose V. Castell, and Maria Teresa Donato 22. The Use of Human Hepatocytes to Investigate Bile Acid Synthesis Ewa C. S. Ellis and Lisa-Mari Nilsson 23. The Use of Primary Human Hepatocytes to Investigate Blood Coagulation Factor Secretion and Activity Christine Biron-Andreani, Edith Raulet, Lydiane Pichard-Garcia, and Patrick Maurel 24. The Use of Human Hepatocytes to Investigate HCV Infection Lydiane Pichard-Garcia, Philippe Briolotti, Dominique Larrey, Antonio Sa-Cunha, Bertrand Suc, Sylvain Laporte, and Patrick Maurel 25. The Use of Hepatocytes to Investigate HDV Infection: The HDV/HepaRG Model Camille


From the reviews:"Hepatocytes presents a well matched mix of theory and practice. ... A very welcome general theme throughout the book are the detailed practical aspects. Some chapters might almost be training manuals in this respect. ... I found the book very hard to criticise To me, it was the ideal textbook ... . Moreover each chapter was written by contributors that dripped with experience. ... the quality of the text is so outstanding." (Graham Lappin, BTS Newsletter, Issue 37, Winter, 2010)"Eighty authors have collaborated to construct 29 chapters ... . the quality of the paper, the layout and print size, and the overall construction of the book are excellent. The 553 pages are abundantly illustrated and annotated with 1591 references followed by a 19-page index ... . The work is a worthy and elegant addition to their 'Methods in Molecular Biology' series and would be equally at home, and useful, around the laboratory, on the office shelf or in a reference library." (S. C. Mitchell, ISSX Newsletter, February, 2011)

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