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High Throughput Screening

In the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche area of study to a major, essential scientific technique. In High Throughput Screening: Methods and Protocols, Second Edition, experts in the field fully update the previous edition to best serve as an introduction to HTS for scientists who are just entering the field, and as a provider of key, significant detail so as to be useful to scientists in established HTS operations. Featuring new screening technologies as well as many well established methods, chapters offer comprehensive treatment of the activities directly related to HTS, such as compound library management, data handling, and robotics. As a volume in the highly successful Methods in Molecular Biology (TM) series, this work provides the kind of meticulous description and implementation advice that is crucial for getting optimal results. Authoritative and intrinsically practical, High Throughput Screening: Methods and Protocols, Second Edition promises to share the highest knowledge and enthusiasm with all those who use this versatile technology in their own labs.
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Table of Contents

1. Design and Implementation of High Throughput Screening Assays Ricardo Macarron and Robert P. Hertzberg 2. Creation of a Small High-Throughput Screening Facility Tod Flak 3. Informatics in Compound Library Management Mark Warne and Louise Pemberton 4. Statistics and Decision Making In High Throughput Screening Isabel Coma, Jesus Herranz, and Julio Martin 5. Enzyme Assay Design for High Throughput Screening Kevin P. Williams and John E. Scott 6. Application of Fluorescence Polarization in HTS Assays Xinyi Huang and Ann Aulabaugh 7. Screening G-Protein Coupled Receptors: Measurement of Intracellular Calcium Using the Fluorometric Imaging Plate Reader Renee Emkey and Nancy B. Rankl 8. High Throughput Automated Confocal Microscopy Imaging Screen of a Kinase Focused Library to Identify p38 Mitogen Activated Protein Kinase Inhibitors Using the GE InCell 3000 Analyzer O. Joseph Trask, Jr., Debra Nickischer, Audrey Baker, Rhonda Gates Williams, Ramani Kandasamy, Patricia A. Johnston, and Paul A. Johnston 9. Recent Advances in Electrophysiology Based Screening Technology and the Impact upon Ion Channel Discovery Research Andrew Southan and Gary Clark 10. Automated Patch Clamping Using the QPatch Kenneth A. Jones, Nicoletta Garbati, Hong Zhang, and Charles H. Large 11. High Throughput Screening of the Cyclic AMP-Dependent Protein Kinase (PKA) Using the Caliper Microfluidic Platform Leonard J. Blackwell, Steve Birkos, Rhonda Hallam, Gretchen Van De Carr, Jamie Arroway, Carla M. Suto, and William P. Janzen 12. Use of Primary Human Cells in High Throughput Screens Mike Jowett, Angela Dunne, and Stephen Rees

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