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The Immunoassay Handbook


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Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing. Please see for a free downloadable chapter (Immunoassay For Beginners), and access to chapter abstracts, keywords, contributor biographies, and updates

Table of Contents

Part 1 IMMUNOASSAY FUNDAMENTALS 1.1 How to use this book 1.2 Immunoassays for beginners 1.3 Immunoassay performance measures Part 2 IMMUNOASSAY CONFIGURATIONS 2.1 Competitive and immunometric assays (including ELISA) 2.2 Non-competitive immunoassays for small molecules 2.3 Homogenous immunoassays 2.4 Lateral flow immunoassay devices 2.5 Ambient analyte immunoassay 2.6 Free analyte immunoassay 2.7 Qualitative immunoassays 2.8 Immunoassays for the detection or quantification of antibodies 2.9 Microsphere-based Multiplex Immunoassays: Development and Applications using Luminex xMAP Technology 2.10 Lab on a chip 2.11 Immunological Biosensors 2.12 SPR 2.13 Single Molecule Array Part 3 IMMUNOASSAY COMPONENTS 3.1 Antibodies 3.2 Signal generation systems 3.3 Solid-phase and other separation systems 3.4 Conjugation methods 3.5 Standardisation and calibration 3.6 Calibration curve-fitting Part 4 RELATED TECHNIQUES 4.1 Foundations of immunochemistry 4.2 Immunohistochemistry and immunofluorescence Part 5 IMMUNOASSAY DEVELOPMENT 5.1 Practical guide to ELISA development 5.2 Performance evaluation and validation 5.3 Interferences in immunoassay 5.4 Immunoassay development in industry Part 6 IMMUNOASSAY IMPLEMENTATION 6.1 Subject preparation, sample collection and handling 6.2 Laboratory quality assurance 6.3 Point-of-care testing 6.4 Choosing an automated immunoassay system 6.5 Troubleshooting guide Part 7 IMMUNOASSAY PRODUCT TECHNOLOGY 7.1 Introduction to product technology 7.2 Market trends 7.3 Lateral Flow and Consumer Diagnostics 7.4 Clearblue 7.5 TRIAGE 7.6 Stratus CS 7.7 IMx 7.8 AxSYM 7.9 Architect 7.10 Advia CENTAUR 7.11 IMMULITE and IMMULITE 1000 7.12 IMMULITE 2000 and IMMULITE 2000 Xpi 7.13 Eci 7.14 Vitros Clin chem 7.15 Access 2 7.16 Dxl 600 and 800 7.17 Elecsys 7.18 PRISM 7.19 Phadia systems Part 8 IMMUNOASSAY APPLICATIONS OTHER THAN CLINICAL CHEMISTRY 8.1 Immunoassay applications in veterinary diagnostics 8.2 Immunoassays in the pharmaceutical industry Part 9 IMMUNOASSAY CLINICAL APPLICATIONS (HUMAN) 9.1 Clinical concepts 9.2 Thyroid 9.3 The adrenal cortex 9.4 Bone metabolism 9.5 Infertility 9.6 In vitro fertilization and embryo transfer 9.7 Hirsutism and virilization in the female 9.8 Pregnancy 9.9 Growth and growth hormone deficiency 9.10 Diabetes mellitus 9.11 Hematology 9.12 Cardiac markers 9.13 Cancer markers 9.14 Allergy 9.15 Autoimmune diseases 9.16 GI Tract 9.17 Hepatitis 9.18 HIV 9.19 Other viral diseases (excl HIV and Hepatitis) 9.20 Other bacterial diseases 9.21 Fungal and parasitic diseases 9.22 Therapeutic drug monitoring 9.23 Drugs of abuse

About the Author

David Wild, 57, is a veteran of the healthcare industry. His experience encompasses pharmaceuticals, medical devices and, for 23 years, immunodiagnostics, which remains his passion. His specialty is products and processes that involve advanced biochemistry and engineering. He worked for Amersham, Eastman-Kodak, Johnson & Johnson and Bristol-Myers Squibb and consulted for diagnostics and biotechnology companies. He worked on the development of Amerlite and Vitros ECi and many new immunoassay chemistries, and has managed the design and construction of new manufacturing facilities. Recent positions include Director of Research and Development, Director of Design Engineering, and Director of Global Operations. He is currently Director of Global Manufacturing Strategy at ConvaTec, a private equity owned medical device company.


"The book starts out very basic with principles of immunoassays ('immunoassays for beginners') and builds progressively to discuss immunoassay components, configurations, development, implementation, product technology, applications other than clinical chemistry, and concludes with 23 chapters on clinical applications to human disease.This is the single 'go-to' book for any questions regarding immunoassays. If this is an area of interest for you, you must have this edition.", November 8, 2013 "Editors Wild, John, Sheehan, Binder, and He present this handbook on the highly sensitive technique of immunoassay.Equations, figures, and real-world examples are used to clarify mechanisms and analysis. The range of detail makes it appropriate as both a pedagogical resource and a reference for immunology labs."--Reference & Research Book News, October 2013 " must be stated that there is much of value in the volume [2nd Ed.]. It is a useful and informative addition to any scientific library."--Robert F. Ritchie, Foundation for Blood Research, Scarborough, USA

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