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A Laboratory Course in Biomaterials
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Table of Contents

Basic Laboratory Skills

Commonly Used Lab Equipment and Supplies

Waste Disposal

Liquid Transfer

Weighing

Making Solutions

Error Analysis

Module I. Drug Delivery: Controlled Release of Encapsulated Protein from PLGA Microspheres

Session 1. Encapsulation of BSA in PLGA Microspheres

Session 2. Evaluation of the Encapsulation Efficiency and the Drug Load

Session 3. Controlled Release of BSA from PLGA Microspheres

Session 4. Evaluation of Controlled Release Profile

Questions

Appendix. Recipes and Sources for Equipment, Reagents, and Supplies

Module II. Natural Biomaterials: Collagen and Chitosan

Session 1. Extracting Acid-Soluble Type I Collagen from Bovine Calf Skin

Session 2. Purification of Extracted Collagen

Session 3. Analysis of Collagen Extraction and Purification by Electrophoresis

Session 4. Fabricating Collagen/Chitosan Sponges

Questions

Appendix. Recipes and Sources for Equipment, Reagents and Supplies

Basic Laboratory Skills II. Cell Culture and Microscopy

Cell Culture

Exercises

Appendix. Sources and Recipes for Equipment, Reagents, and Supplies

Module III. Biocompatibility Testing: Cytotoxicity and Adhesion

Session 1. Cytotoxicity Evaluation Using Direct Contact Tests

Session 2. Cytotoxicity Evaluation Using Liquid Extracts of Materials

Session 3. Biofunctionality Evaluation through Cell Adhesion/Proliferation Tests

Questions

Appendix. Recipes and Sources for Equipment, Reagents and Supplies

Module IV. Tissue Engineering: Organotypic Cultures of Skin Equivalents

Session 1. The Dermis Equivalent

Session 2. The Epidermis Equivalent

Session 3. Histological Studies of Natural Skin and Organotypic Skin Equivalent

Questions

Appendix A. Sterilizing Collagen and Preparing Collagen Solution

Appendix B. Custom-Made Teflon Support for Air Exposure of the Epidermis Equivalent

Appendix C. Recipes and Sources for Equipment, Reagents and Supplies

Module V. Bioceramics: Porous Hydroxyapatite Composite

Session 1. Synthesis of HA and FA

Session 2. X-Ray Powder Diffraction of HA and FA

Session 3. Fabrication of Porous HA/FA-PLGA Composite Using a Salt-Leaching Method

Session 4. Characterization of the Porous HA/FA-PLGA Composites-Acid Degradation and Compression Testing

Questions

Appendix. Recipes and Sources for Equipment, Reagents and Supplies

Appendix: Answers

Index

About the Author

Wujing Xian is a professor at the University of Illinois at Urbana-Champaign.

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