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Table of Contents

1. ENU Based Phenotype Driven Screening Vera M. Ripoll, Philip L. Kong, and Paul K. Potter 2. Detection and Quantification of Cytokines and Other Biomarkers Evan L. Chiswick, Elizabeth Duffy, Brian Japp and Daniel Remick 3. Flow Cystometry Analysis of Cell Cycling and Proliferation in Mouse Hematopoetic Stem and Progenitor Cells Valerie Barbier, Bianca Nowlan, Jean-Pierre Levesque and Ingrid G. Winkler 4. Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow in Vivo Valerie Barbier, Ingrid G. Winkler, Robert Wadley and Jean-Pierre Levesque 5. Analyzing Cell Death Events in Cultured Leukocytes Karin Christenson, Fredrik B. Thoren and Johan Bylund 6. Towards a Four-Dimensional View of Neutrophils Ben A. Croker, Andrew W. Roberts and Nicos A. Nicola 7. Isolation of Human and Mouse Neutrophils Ex vivo and In vitro Yan Hu 8. Measurement of Oxidative Burst in Neutrophils Yu Chen and Wolfgang G. Junger 9. Measurement of Neutrophil Elastase, Proteinase 3 and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic SubstratesBrice Korkmaz, Sylvie Attucci, Christophe Epinette, Elodie Pitois, Marie-Lise Jourdan, Luiz Juliano and Francis Gauthier 10. The Macrophage C.P. Verschoor, A. Puchta and D.M.E. Bowdish 11. Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice R. Tedjo Sasmono and Elizabeth Williams 12. Generation of Mouse Bone Marrow-derived Macrophages Silvia Manzanero 13. Isolation and Differentiation of Monocytes-macrophages from Human Blood Dipti Vijayan 14. In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages Andre Moraes Nicola and Arturo Casadevall 15. Measuring the Inflammasome Olaf Gross 16. Arginine and Macrophage Activation Monica Comalada, Andree Yeramian, Manuel Modolell, Jorge Lloberas and Antonio Celada 17.Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation Catherina H. Bird,Corrine Hitchen , Mark Prescott, Ian Harper and Phillip I. Bird 18. Detection of Human and Mouse Granzyme B Activity in Cell Extracts Sarah Elizabeth Stewart, Matthew Stephen James Mangan, Phillip Ian Bird and Dion Kaiserman 19. T cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T cells in Chronic Intestinal Inflammation Rajaraman Eri, Michael A. McGuckin and Robert Wadley 20. Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry Dimitrios Tsikas, Maria-Theresia Suchy, Anja, Bibiana Beckmann and Frank-Mathias Gutzki

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