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Leucocytes: Methods and Protocols (Methods in Molecular Biology)

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Methods and Protocols (Methods in Molecular Biology)

By Robert B. Ashman (Edited by)

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Format: Paperback, 296 pages, Softcover reprint of Edition
Other Information: 12 Tables, black and white; XI, 296 p.
Published In: United States, 23 August 2016
The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. In, Leucocytes: Methods and Protocols provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Leucocytes: Methods and Protocols seeks to aid scientists in further study into the immune system and leucocytes.

Table of Contents

1. ENU Based Phenotype Driven ScreeningVera M. Ripoll, Philip L. Kong, and Paul K. Potter2. Detection and Quantification of Cytokines and Other BiomarkersEvan L. Chiswick, Elizabeth Duffy, Brian Japp and Daniel Remick3. Flow Cystometry Analysis of Cell Cycling and Proliferation in Mouse Hematopoetic Stem and Progenitor Cells Valerie Barbier, Bianca Nowlan, Jean-Pierre Levesque and Ingrid G. Winkler4. Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow in VivoValerie Barbier, Ingrid G. Winkler, Robert Wadley and Jean-Pierre Levesque5. Analyzing Cell Death Events in Cultured LeukocytesKarin Christenson, Fredrik B. Thoren and Johan Bylund6. Towards a Four-Dimensional View of NeutrophilsBen A. Croker, Andrew W. Roberts and Nicos A. Nicola7. Isolation of Human and Mouse Neutrophils Ex vivo and In vitroYan Hu8. Measurement of Oxidative Burst in NeutrophilsYu Chen and Wolfgang G. Junger9. Measurement of Neutrophil Elastase, Proteinase 3 and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic SubstratesBrice Korkmaz, Sylvie Attucci, Christophe Epinette, Elodie Pitois, Marie-Lise Jourdan, Luiz Juliano and Francis Gauthier10. The MacrophageC.P. Verschoor, A. Puchta and D.M.E. Bowdish11. Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic MiceR. Tedjo Sasmono and Elizabeth Williams12. Generation of Mouse Bone Marrow-derived MacrophagesSilvia Manzanero13. Isolation and Differentiation of Monocytes-macrophages from Human BloodDipti Vijayan14. In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by MacrophagesAndre Moraes Nicola and Arturo Casadevall15. Measuring the InflammasomeOlaf Gross16. Arginine and Macrophage ActivationMonica Comalada, Andree Yeramian, Manuel Modolell, Jorge Lloberas and Antonio Celada17.Immunodetection of Granzyme B Tissue Distribution and Cellular LocalisationCatherina H. Bird,Corrine Hitchen , Mark Prescott, Ian Harper and Phillip I. Bird18. Detection of Human and Mouse Granzyme B Activity in Cell Extracts Sarah Elizabeth Stewart, Matthew Stephen James Mangan, Phillip Ian Bird and Dion Kaiserman19. T cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T cells in Chronic Intestinal InflammationRajaraman Eri, Michael A. McGuckin and Robert Wadley20. Measurement of Nitrite in Urine by Gas Chromatography-Mass SpectrometryDimitrios Tsikas, Maria-Theresia Suchy, Anja, Bibiana Beckmann and Frank-Mathias Gutzki

EAN: 9781493962310
ISBN: 1493962310
Publisher: Humana Press Inc.
Dimensions: 25.4 x 17.8 centimetres (0.59 kg)
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