Table of Contents

1. Milestones in the development of liquid chromatography
2. Kinetic theories of liquid chromatography
3. Column technology in liquid chromatography
4. General instrumentation in HPLC
5. Liquid-solid chromatography
6. Reversed-phase liquid chromatography
7. Secondary chemical equilibria in reversed-phase liquid chromatography
8. Ultra-high performance and Ultrafast liquid chromatography
9. Nano-liquid chromatography
10. Hydrophilic interaction liquid chromatography
11. Mobile phase selection in liquid chromatography
12. Co-solvents and mobile phase additives (new chapter)
13. Method development in liquid chromatography
14. Physicochemical measurements
15. Theory and practice of gradient elution liquid chromatography
16. Fundamentals of Enantioselective LC (Chiral recognition mechanisms)
17. Hydrophobic interaction chromatography
18. Ion chromatography
19. Size-exclusion chromatography
20. Interaction polymer chromatography
21. Affinity chromatography
22. Multidimensional Liquid Chromatography
23. Process concepts in preparative chromatography
24. Modeling of preparative liquid chromatography
25. Capillary electrochromatography
26. Liquid Chromatography on microchip
27. Mass spectrometric detection, instrumentation and ionization methods
28. Identification and quantitation in Mass Spectrometry
29. Advanced IR and Raman detectors for identification and quantification
30. Advanced spectroscopic detectors for identification and quantification: Nuclear magnetic resonance
31. Prediction of retention in Liquid Chromatography
32. Validation of liquid chromatographic methods – chemometrics and calibration

About the Author

Salvatore Fanali is Director of Research at the Institute of Chemical Methodologies, Italian National Research Council (C.N.R.) in Monterotondo (Rome), Italy, and head of the Capillary Electromigration and Chromatographic Methods Unit at the same Institute. His research activity is mainly focused on separation science including the development of modern miniaturized techniques (electrodriven and liquid chromatography). He also studies hyphenation with mass spectrometry and development of new stationary phases. Separation methods developed are currently applied to food, pharmaceuticals, chiral environment, and biomedical analysis. He is Editor of the Journal of Chromatography A and a member of the advisory editorial board of seven international scientific journals. Fanali is the author of about 300 publications including some book chapters. He received several awards including the “Liberti Medal” in Separation Science from the Italian Chemical Society. Bezhan Chankvetadze is professor of Analytical Chemistry at Tbilisi State University, Editor of Journal of Pharmaceutical and Biomedical Analysis, and is well recognized in the field of separation science. He is one of the leaders in chromatography and chiral separations with a large number of publications. Paul Haddad is currently a Distinguished Professor of Chemistry and Australian Research Council Federation Fellow at the University of Tasmania, as well as Director of the Pfizer Analytical Research Centre. He has more than 500 publications in this field and has presented in excess of 450 papers at local and international scientific meetings. He is an editor of Journal of Chromatography A, a contributing editor for Trends in Analytical Chemistry, and was an editor of Analytica Chimica Acta for 6 years. He is currently a member of the editorial boards of 10 other journals of analytical chemistry or separation science.

He is the recipient of several national and international awards, including the ACS Award in Chromatography, the Marcel Golay Award, the AJP Martin Gold Medal awarded by the Chromatographic Society, the Royal Society of Chemistry Analytical Separation Methods Award, the RACI HG Smith and Analytical Division medals, and more. Professor Colin Poole is internationally known in the field of thin-layer chromatography and is an editor of the Journal of Chromatography and former editor of the Journal of Planar Chromatography – Modern TLC. He has authored several books on chromatography, recent examples being The Essence of Chromatography published by Elsevier (2003), and Gas Chromatography published by Elsevier (2012). He is the author of approximately 400 research articles, many of which deal with thin-layer chromatography, and is co-chair of the biennial “International Symposium on High-Performance Thin-Layer Chromatography”. Marja-Liisa Riekkola is a professor of Analytical Chemistry at Helsinki University, Helsinki, Finland. She is well recognized in the field of separation science. She serves as Editor of Journal of Chromatography A. Prof. Riekkola is one of the leaders in chromatography with a large number of publications.