Table of Contents

1: Skelly, Sohi & Batuwangala: Classical Cloning, Expression and Purification
2: Owens, Nettleship, Berrow, Stuart & Stammers: High-Throughput Cloning, Expression and Purification
3: Chayen: Automation of Non-Conventional Crystallization Techniques for Screening and Optimisation
4: Abdel-Meguid, Jeruzalmi & Sanderson: First Analysis of Macromolecular Crystals
5: Sanderson: In-House Macromolecular Data Collection
6: Abdel-Meguid: Solving the Phase Problem Using Isomorphous Replacement
7: Delarue: Molecular Replacement Techniques for High-Throughput Structure Determination
8: Murthy: MAD Phasing
9: Weeks & Furey: Application of Direct Methods to Macromolecular Structure Solution
10: Abrahams: Phase Refinement through Density Modification
11: Morris, Perrakis & Lamzin: Getting a Macromolecular Model: Model Building, Refinement and Validation
12: Wasserman, Smith, D'Amico, Koss, Morisco, and Burley: High-Throughput Crystallographic Data Collection at Synchrotrons
13: Carson: Electron Density Fitting and Structure Validation
14: Masquida, Francois, Werner & Westhof: RNA Crystallogenesis
15: Sohi & Laponogov: Crystallography in the Study of Protein-DNA Interaction
16: Fry, Abrescia & Stuart: Virus Crystallography
17: Abdel-Meguid: Macromolecular Crystallography in Drug Design

About the Author

Mark Sanderson is an experienced protein and nucleic-acid crystallographer and co-editor of Methods in Molecular Biology, who has solved several key ab initio structures, notably nucleic-acid proteins. Jane Skelly has widespread experience in the expression and crystallisation of macromolecules.
Both editors lecture undergraduates in structural biology.

Reviews

it is more than successful, and most, if not all, who dip into it will be rewarded by learning things they did not know, or possibly had forgotten...[..] a book that will surely find its place in every crystallographic lab whether protein or macromolecular.
*Lindsay Sawyer, Crystallography News*