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Manipulating the Early Embryo of Xenopus Laevis

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Equipment for embryo experiments; getting embryos; preparing embryos for manipulation; embryo peturbations; microinjection; lineage labelling; microdissection; method for generating transgenic frog embryos; immunocytochemistry; in situ hybridization; histology. Appendices: nucleic acid and protein methods; culture media; fate maps of blastula stages; morphology of Xenopus embryos and adults; timing of development and temperature dependence.

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