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Manipulating the Early Embryo of Xenopus Laevis
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The large, readily available eggs of Xenopus laevis are a valuable subject for the study of developmental events. This 3-volume video set contains 68 demonstrations of the manipulation of living eggs using techniques taught for four years in the Cold Spring Harbor Laboratory course on Xenopus development. The video was produced using digital techniques to ensure no loss of resolution of the fine images captured by microphotography. Recorded live among the course participants, the videotape accurately reflects the triumphs and tribulations of real investigators as they attempt painstaking procedures while commenting on their actions for the students benefit.

This video guide is an essential learning tool for investigators considering work with Xenopus eggs and embryos.

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Equipment for embryo experiments; getting embryos; preparing embryos for manipulation; embryo peturbations; microinjection; lineage labelling; microdissection; method for generating transgenic frog embryos; immunocytochemistry; in situ hybridization; histology. Appendices: nucleic acid and protein methods; culture media; fate maps of blastula stages; morphology of Xenopus embryos and adults; timing of development and temperature dependence.

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