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Versatile, comprehensive, and clearly written, this competitively priced laboratory manual can be used with any undergraduate microbiology text-and now features a stunning full-color design, integrated color photographs, and newly rendered color illustrations. Microbiology: A Laboratory Manual is known for its thorough coverage, descriptive and straightforward procedures, and minimal equipment requirements. A broad range of experiments helps to convey basic principles and techniques. Each experiment includes an overview, an in-depth discussion of the principle involved, easy-to-follow procedures, and lab reports with review and critical thinking questions. Ample introductory material and laboratory safety instructions are provided.
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Table of Contents

Part 1: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms 1. Culture Transfer Techniques 2. Techniques for Isolation of Pure Cultures 3. Cultural Characteristics of Microorganisms Part 2: Microscopy 4. Microscopic Examination of Stained Cell Preparations 5. Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount 6. The Microscopic Measurement of Microorganisms Part 3: Bacterial Staining 7. Preparation of Bacterial Smears 8. Simple Staining 9. Negative Staining 10. Gram Stain 11. Acid-Fast Stain (Ziehl-Neelsen Method) 12. Differential Staining for Visualization of Bacterial Cell Structures Part 4: Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations 13. Nutritional Requirements: Media for the Routine Cultivation of Bacteria 14. Use of Differential, Selective, and Enriched Media 15. Physical Factors: Temperature 16. Physical Factors: pH of the Extracellular Environment 17. Physical Factors: Atmospheric Oxygen Requirements 18. Techniques for the Cultivation of Anaerobic Microorganisms 19. Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells 20. The Bacterial Growth Curve Part 5: Biochemical Activities of Microorganisms 21. Extracellular Enzymatic Activities of Microorganisms 22. Carbohydrate Fermentation 23. Triple Sugar-Iron Agar Test 24. IMViC Test 25. Hydrogen Sulfide Test 26. Urease Test 27. Litmus Milk Reactions 28. Nitrate Reduction Test 29. Catalase Test 30. Oxidase Test 31. Utilization of Amino Acids 32. Genus Identification of Unknown Bacterial Cultures Part 6: The Protozoa 33. Free-Living Protozoa 34. Parasitic Protozoa Part 7: The Fungi 35. Cultivation and Morphology of Molds 36. Yeast Morphology, Cultural Characteristics, and Reproduction 37. Identification of Unknown Fungi Part 8: The Viruses 38. Cultivation and Enumeration of Bacteriophages 39. Isolation of Coliphages from Raw Sewage Part 9: Physical and Chemical Agents for the Control of Microbial Growth 40. Physical Agents of Control: Moist Heat 41. Physical Agents of Control: Environmental Osmotic Pressure 42. Physical Agents of Control: Electromagnetic Radiation 43. Chemical Agents of Control: Chemotherapeutic Agents 44. Determination of Penicillin Activity in the Presence and Absence of Penicillinase 45. Chemical Agents of Control: Disinfectants and Antiseptics Part 10: Microbiology of Food 46. Microbiological Analysis of Food Products: Bacterial Count 47. Wine Production Part 11: Microbiology of Water 48. Standard Qualitative Analysis of Water 49. Quantitative Analysis of Water: Membrane Filter Method Part 12: Microbiology of Soil 50. Microbial Populations in Soil: Enumeration 51. Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates 52. Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique Part 13: Bacterial Genetics 53. Enzyme Induction 54. Bacterial Conjugation 55. Isolation of a Streptomycin-Resistant Mutant 56. The Ames Test: A Bacterial Test System for Chemical Carcinogenicity Part 14: Biotechnology 57. Bacterial Transformation 58. Isolation of Bacterial Plasmids 59. Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA Part 15: Medical Microbiology 60. Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries 61. Normal Microbial Flora of the Throat and Skin 62. Identification of Human Staphylococcal Pathogens 63. Identification of Human Streptococcal Pathogens 64. Identification of Streptococcus pneumoniae 65. Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems 66. Isolation and Presumptive Identification of Campylobacter 67. Microbiological Analysis of Urine Specimens 68. Microbiological Analysis of Blood Specimens 69. Species Identification of Unknown Bacterial Cultures Part 16: Immunology 70. Precipitin Reaction: The Ring Test 71. Agglutination Reaction: The Febrile Antibody 72. Immunofluorescence 73. Enzyme-Linked Immunoabsorbent Assay 74. Agglutination Reaction: Mono-Test for Infectious Mononucleosis 75. Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures Appendices

About the Author

James Cappuccino is a retired instructor from the State University of New York (SUNY) system at Rockland Community College.

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