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Table of Contents

Part 1: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms1. Effectiveness of Hand Washing2. Culture Transfer Techniques3. Techniques for Isolation of Pure Cultures4. Cultural Characteristics of Microorganisms Part 2: Microscopy5. Microscopic Examination of Stained Cell Preparations6. Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount7. The Microscopic Measurement of Microorganisms Part 3: Bacterial Staining8. Preparation of Bacterial Smears9. Simple Staining10. Negative Staining11. Gram Stain12. Acid-Fast Stain 13. Differential Staining for Visualization of Bacterial Cell Structures Part 4: Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations14. Nutritional Requirements: Media for the Routine Cultivation of Bacteria15. Use of Differential, Selective, and Enriched Media16. Physical Factors: Temperature17. Physical Factors: pH of the Extracellular Environment18. Physical Factors: Atmospheric Oxygen Requirements19. Techniques for the Cultivation of Anaerobic Microorganisms20. Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells21. The Bacterial Growth Curve Part 5: Biochemical Activities of Microorganisms22. Extracellular Enzymatic Activities of Microorganisms23. Carbohydrate Fermentation24. Triple Sugar-Iron Agar Test25. IMViC Test26. Hydrogen Sulfide Test27. Urease Test28. Litmus Milk Reactions29. Nitrate Reduction Test30. Catalase Test31. Oxidase Test32. Utilization of Amino Acids33. Genus Identification of Unknown Bacterial Cultures Part 6: The Protozoa34. Free-Living Protozoa35. Parasitic Protozoa Part 7: The Fungi36. Cultivation and Morphology of Molds37. Yeast Morphology, Cultural Characteristics, and Reproduction38. Identification of Unknown Fungi Part 8: The Viruses39. Cultivation and Enumeration of Bacteriophages40. Isolation of Coliphages from Raw Sewage Part 9: Physical and Chemical Agents for the Control of Microbial Growth41. Physical Agents of Control: Moist Heat42. Physical Agents of Control: Environmental Osmotic Pressure43. Physical Agents of Control: Electromagnetic Radiations44. Chemical Agents of Control: Chemotherapeutic Agents45. Determination of Penicillin Activity in the Presence and Absence of Penicillinase46. Chemical Agents of Control: Disinfectants and Antiseptics Part 10: Microbiology of Food 47. Microbiological Analysis of Food Products: Bacterial Count48. Wine Production Part 11: Microbiology of Water49. Standard Qualitative Analysis of Water50. Quantitative Analysis of Water: Membrane Filter Method Part 12: Microbiology of Soil51. Microbial Populations in Soil: Enumeration52. Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates53. Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique Part 13: Bacterial Genetics53. Enzyme Induction54. Bacterial Conjugation55. Isolation of a Streptomycin-Resistant Mutant56. The Ames Test: A Bacterial Test System for Chemical Carcinogenicity Part 14: Biotechnology57. Bacterial Transformation58. Isolation of Bacterial Plasmids59. Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA Part 15: Medical Microbiology60. Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries61. Normal Microbial Flora of the Throat and Skin62. Identification of Human Staphylococcal Pathogens63. Identification of Human Streptococcal Pathogens64. Identification of Streptococcus pneumoniae65. Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems66. Isolation and Presumptive Identification of Campylobacter67. Microbiological Analysis of Blood Specimens68. Microbiological Analysis of Urine Specimens69. Species Identification of Unknown Bacterial Cultures Part 16: Immunology70. Precipitin Reaction: The Ring Test71. Agglutination Reaction: The Febrile Antibody72. Immunofluorescence73. Enzyme-Linked Immunoabsorbent Assay74. Agglutination Reaction: Mono-Test for Infectious Mononucleosis75. Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures Appendices

About the Author

James Cappuccino is a Professor Emeritus at the State University of New York (SUNY) system at Rockland Community College.

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