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Molecular Cloning


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Chapter 1. Plasmids and Their Usefulness in Molecular Cloning Chapter 2. Bacteriophage lambda and Its Vectors Chapter 3. Working with Bacteriophage M13 Vectors Chapter 4. Working with High-capacity Vectors Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction Chapter 9. Preparation of Radiolabeled DNA and RNA Probes Chapter 10. Working with Synthetic Oligonucleotide Probes Chapter 11. Preparation of cDNA Libraries and Gene Identification Chapter 12. DNA Sequencing Chapter 13. Mutagenesis Chapter 14. Screening Expression Libraries Chapter 15. Expression of Cloned Genes in Escherichia coli Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells Chapter 18. Protein Interaction Technologies Appendices


"In every kitchen there is at least one indispensable cookbook. Sambrook and Russell's Molecular Cloning: A Laboratory Manual fills the same niche in the laboratory. Like its kitchen counterparts (e.g. Rombeck's Joy of Cooking) Sambrook's Molecular Cloning (MC) has information to help both the inexperienced and the advanced user." --Trends in Neurosciences

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