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Multidimensional Liquid Chromatography

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Table of Contents

Preface. 1. Introduction, (Mark R. Schure and Steven A. Cohen). Theory. 2. Elements of the Theory of Multidimensional Liquid Chromatography, (Mark R. Schure). 3. Peak capacity in Two-dimensional Liquid Chromatography (Joe M. Davis). 4. Decoding Complex 2-D Separations (Francseso Dondi, Maria Chiara Pietrogrande, Nicola Marchetti and Attila Felinger). Columns, Instrumentation, and Methods. 5. Instrumentation for Comprehensive Multidimensional Liquid Chromatography (Robert. E. Murphy and Mark R. Schure). 6. Methods Development for Comprehensive Multidimensional Liquid Chromatography (Robert E. Murphy and Mark R. Schure). 7. Monolithic Columns and their 2D-HPLC applications (Tohru Ikegami, Hiroshi Aoki, Hiroshi Kimura, Ken Hosoya and Nobuo Tanaka). 8. Ultra-High Pressure Multidimensional Liquid Chromatography (Charles R. Evans and James W. Jorgenson). Life Sciences Applications. 9. Peptidomics (Egidijus Machtejevas, Klaus K. Unger). 10. A Two-dimensional Liquid Mass Mapping Technique for Biomarker (David Lubman, Nathan S. Buchanan, Fred R. Miller, Kathleen Cho, Rong Wu, Steven Goodison, Yanfei Wang, Paweena Kreunin and Timothy J. Barder). 11. Coupled Multidimensional Chromatography and Tandem Mass Spectrometry Systems for Complex Peptide Mixture Analysis (Mike Washburn). 12. Development of Orthogonal 2D-LC Methods for Separations of Peptides (Martin Gilar, Petra Olivova, Amy E. Daly and John C. Gebler). 13. Protein IEX-RP-TOF MS (Steven A. Cohen and Scott Berger). 14. Analysis of Enantiomeric Compounds Using Multidimensional Liquid Chromatography (Renee J. Soukup and Dan Armstrong). Multidimensional separations using CE. 15. Two-dimensional capillary electrophoresis for the comprehensive analysis of complex protein mixtures (James R. Kraly, Melissa M. Harwood, Megan Jones and Norman J. Dovici). 16. Two-dimensional HPLC-CE methods for protein/Peptide separation (Haleem Issaq). Industrial Applications. 17. Multidimensional Liquid Chromatography in Industrial Applications (Frank Rittig and Harald Pasch). 18. The Analysis of Surfactants by Multidimensional Liquid Chromatography (Robert E. Murphy and Mark R. Schure.

About the Author

Steven A. Cohen, PhD, is Life Sciences Director, RDE of the Proteomics Technology Group at the Waters Corporation in Milford, Massachusetts. MARK R. SCHURE, PhD, is Technical Director of the Computational Chemistry Group and Technical Director of the Theoretical Separation Science Laboratory at the Rohm and Haas Company in Springhouse, Pennsylvania.


"It is a timely publication and present a valuable resource of scientific information on MDLC...It presents systematically gathered scientific information from a plethora of articles scattered over a wide range of sources. This effort should be appreciated by a wide audience of scientists and researchers who deal with complex separation programs in biomedical, environmental, and natural products; industrial polymers; food and other sources." ( Journal of the American Chemical Society , November 12, 2008)

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