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Neutrophil Methods and Protocols


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Part I. Neutrophils: an Overview 1. The Role of Neutrophils in the Immune System: An Overview Harry L. Malech, Frank R. DeLeo, and Mark T. Quinn Part II. Neutrophil Isolation and Subcellular Fractionation 2. Isolation of Human Neutrophils William M. Nauseef 3. Neutrophil Isolation from Non-Human Species Daniel W. Siemsen, Natalia Malachowa, Igor A. Schepetkin, Adeline R. Whitney, Lilya N. Kirpotina, Benfang Lei, Frank R. DeLeo, and Mark T. Quinn 4. Collection of In vivo Transmigrated Neutrophils from Human Skin Karin Christenson, Lena Bjoerkman, Lisa Davidsson, Anna Karlsson, Per Follin, Claes Dahlgren, and Johan Bylund 5. Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers Stine Novrup Clemmensen, Lene Udby, and Niels Borregaard Part III. Biochemistry, Biology and Signal Transduction of Neutrophils 6. Rho Family and Rap GTPase Activation Assays Richard T. Jennings and Ulla G. Knaus 7. Measurement of Phospholipid Metabolism in Intact Neutrophils Susan Sergeant and Linda C. McPhail 8. Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium Signals in Neutrophils Maurice B. Hallett , Maha Al-Jumaa, and Sharon Dewitt 9. Analysis of Electrophysiological Properties and Responses of Neutrophils Deri Morgan and Thomas E. DeCoursey 10. Analysis of Neutrophil Apoptosis David A. Dorward, Adriano G. Rossi, Ian Dransfield, and Christopher D. Lucas 11. Microinjection Methods for Neutrophils Iraj Laffafian, Kimberly J. Lewis, K. Benjamin Masterman, and Maurice B. Hallett 12. Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells Colin L. Sweeney, Randall K. Merling, Uimook Choi, Debra Long Priel, Douglas B. Kuhns, Hongmei Wang, and Harry L. Malech Part IV. Neutrophil Adhesion and Chemotaxis 13. Neutrophil Migration Through Extracellular Matrix Richard T. Jennings and Ulla G. Knaus 14. Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafish Pui-ying Lam, Robert S. Fischer, William D. Shin, Clare M. Waterman, and Anna Huttenlocher 15. Detection of Bi-Directional Signalilng During Integrin Activation and In Neutrophil AdhesionStuart M. Altman, Neha Dixit, and Scott I. Simon Part V. Neutrophil Phagocytosis and Bactericidal Activity 16. Immunofluorescence and Confocal Microscopy of Neutrophils Lee-Ann H. Allen 17. Expression of Genetically-Encoded Fluorescent Probes to Monitor Phospholipid Dynamics in Live Neutrophils Benjamin E. Steinberg, Marco A.O. Magalhaes, and Sergio Grinstein 18. Quantitative Assessment of Neutrophil Phagocytosis Using Flow Cytometry Pontus Nordenfelt 19. Analysis of Neutrophil Bactericidal Activity Heather A. Parker, Nicholas J. Magon, Jessie N. Green, Mark B. Hampton, and Christine C. Winterbourn 20. Induction and Quantification of Neutrophil Extracellular Traps Alejandro Sanchez Gonzalez, Bart W. Bardoel, Christopher J. Harbort, and Arturo Zychlinsky Part VI. NADPH Oxidase and Production of Reactive Oxygen Species 21. Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes Johan Bylund, Halla Bjoernsdottir, Martina Sundqvist, Anna Karlsson, and Claes Dahlgren 22. Cell-free NADPH Oxidase Assays: "In vitro Veritas" Edgar Pick 23. Assessment of Priming of the Human Neutrophil Respiratory Burst Margarita Hurtado-Nedelec, Karama Makni-Maalej, Marie-Anne Gougerot-Pocidalo, Pham My-Chan Dang, and Jamel El-Benna 24. Affinity Purification and Reconstitution of Human Phagocyte Flavocytochrome b for Detection of Conformational Dynamics in the Membrane Marcia Riesselman and Algirdas J. Jesaitis 25. Evaluation of p47phox Phosphorylation in Human Neutrophils Using Phospho-Specific Antibodies Sahra Amel Belambri, Pham My-Chan Dang, and Jamel El-Benna Part VII. Analysis of Neutrophil Gene Expression and Transcription Factors 26. Genome-Scale Transcript Analyses with Human Neutrophils Scott D. Kobayashi, Daniel E. Sturdevant, and Frank R. DeLeo 27. Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni 28. High Purity Neutrophil Isolation from Human Peripheral Blood and Saliva for Transcriptome Analysis Flavia S. Lakschevitz and Michael Glogauer 29. Detection of Intact Transcription Factors in Human Neutrophils Patrick P. McDonald and Richard D. Ye Part VIII. Neutrophil Defects and Diagnosis 30. Disorders of Neutrophil Function: An Overview Mary C. Dinauer 31. Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders Houda Zghal Elloumi and Steven M. Holland 32. Diagnostic Assays for Myeloperoxidase and Myeloperoxidase Deficiency William M. Nauseef

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