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Organelles in Eukaryotic Cells

Every year, the Federation of European Biochemical Societies sponsors a series of Advanced Courses designed to acquaint postgraduate students and young postdoctoral fellows with theoretical and practical aspects of topics of current interest in biochemistry, particularly within areas in which significant advances are being made. This volume contains the Proceedings of FEBS Advanced Course No. 88-02 held in Bari, Italy on the topic "Organelles of Eukaryotic Cells: Molecular Structure and Interactions. " It was a deliberate decision of the organizers not to restrict FEBS Advanced Course 88-02 to a discussion of a single organelle or a single aspect but to cover a broad area. One of the objectives of the course was to compare different organelles in order to allow the participants to discern recurrent themes which would illustrate that a basic unity exists in spite of the diversity. A second objective of the course was to acquaint the participants with the latest experimental approaches being used by in- vestigators to study different organelles; this would illustrate that methodologies developed for studying the biogenesis of the structure-function relationships in one organelle can often be applied fruitfully to investi- gate such aspects in other organelles. A third objective was to impress upon the participants that a study of the interaction between different organelles is intrinsic to understanding their physiological functions. This volume is divided into five sections. Part I is entitled "Structure and Organization of Intracellular Organelles.
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Table of Contents

I: Structure and Organization of Intracellular Organelles.- The Mitochondrial Respiratory Chain.- The F0F1 H+-ATP Synthase of Mitochondria.- Functions, Biogenesis and Pathology of Peroxisomes in Man.- II: Intracellular Traffic of Componenents of Organelles.- Membrane Flow and Endocytosis.- Transport of Lipids and Proteins during Membrane Flow in Eukaryotic Cells.- III: Interactions Between Intracellular Organelles.- Control Analysis: Principles and Application to Experimental Practice.- Cellular Organization of Nitrogen Metabolism.- On the Cellular Mechanism of Action of Diphtheria Toxin.- IV: Genetic Interactions.- Organization and Evolution of Mitochondrial DNA in Metazoa.- Tissue-Specific Expression of Nuclear Genes for Mitochondrial Enzymes.- The Plastid Envelope Membranes: Purification, Composition and Role in Plastid Biogenesis.- Biogenesis of Peroxisomes.- Biogenesis and Evolutionary Origin of Peroxisomes.- V: Methodological Section.- Functional and Immunological Characterization of Mitochondrial F0F1 ATP-Synthase.- The Mitochondrial b-c1 Complex: Polypeptide Composition and Enzymatic Activities.- In Vitro Synthesis and Import of Mitochondrial Proteins.- Control of Lipogenesis in Maintenance Cultures of Isolated Hepatocytes.- Enzymic Reactions in Ureogenesis: Analysis of the Control of Citrulline Synthesis in Isolated Rat-Liver Mitochondria.- Chromaffin Granules and Ghosts: Isolation and Measurement of Electrochemical Proton Gradient and Amine Uptake.- Contributors.

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