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Phage Display


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SECTION 1 - PHAGE DISPLAY Chapter 1. Filamentous Phage Biology Chapter 2. Phage-display Vectors Chapter 3. Antibody Libraries Chapter 4. Peptide Libraries Chapter 5. Functional Domains and Scaffolds Chapter 6. Gene Fragment Libraries and Genomic and cDNA Expression Cloning SECTION 2 - ANTIBODY LIBRARIES Chapter 7. Overview: Amplification of Antibody Genes Chapter 8. Generation of Antibody Libraries: Immunization, RNA Preparation, and cDNA Synthesis Chapter 9. Generation of Antibody Libraries: PCR Amplification and Assembly of Light- And Heavy-chain Coding Sequences Chapter 10. Selection from Antibody Libraries Chapter 11. Analysis of Selected Antibodies Chapter 12. Production and Purification of Fab and scFv Chapter 13. Antibody Engineering SECTION 3 - PEPTIDE LIBRARIES Chapter 14. Overview: Peptide Libraries Chapter 15. General Phage Methods Chapter 16. Production of Peptide Libraries Chapter 17. Screening Peptide Libraries Chapter 18. Analysis of Phage-borne Peptides Chapter 19. Construction and Use of pIII-displayed Peptide Libraries SECTION 4 - OTHER LIBRARIES AND OTHER METHODS OF PANNING Chapter 20. Construction and Selection from Gene Fragment Phage-display Expression Libraries Chapter 21. Construction and Selection from cDNA Phage-display Expression Libraries Chapter 22. In Vivo Selection of Phage-display Libraries Chapter 23. Cell-surface Selection and Analysis of Monoclonal Antibodies from Phage Libraries SECTION 5 - APPENDICES Appendix 1. Useful Information Appendix 2. Recipes Appendix 3. General Procedures Appendix 4. Cautions Appendix 5. Suppliers Appendix 6. Trademarks


The book's raison d' ea;tre is as a source of detailed protocols. These are clearly written, and include ideas for experimental controls and useful trouble-shooting notes. Jargon is generally avoided, and the protocols are up to date. ...[This] is a much-needed and well-executed attempt at theoretical and practical support to any researcher, established or novice, using phage display. Trends in Genetics This book is a solid work that addresses both theoretical and practical aspects of phage display technology. It covers the use of phage display in protein engineering with particular emphasis on antibody, peptide, cDNA libraries and ligand receptor engineering, which together represent [approximately] 90% of the phage display field. Anyone using or contemplating any of these applications would find it worthwhile to read the relevant chapters. Trends in Biotechnology

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