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Phosphorous-31 NMR

Phosphorous-31 NMR: Principles and Applications focuses on the evolution of phosphorus-31 nuclear magnetic resonance (NMR) as a widely applied spectroscopic probe of the structure and dynamics of phosphorus-containing compounds. The selection first offers information on the principles and empirical observations of phosphorus-31 chemical shifts and the principles and applications of phosphorus-31 spin-spin coupling constants. Discussions focus on introduction and basic principles, directly bonded phosphorus coupling constants, and two-bond and three-bond coupling constants. The text then ponders on phosphorus-31 NMR of enzyme complexes and paramagnetic probes of enzyme complexes with phosphorus-containing compounds. The manuscript elaborates on the use of chiral thiophosphates and the stereochemistry of enzymatic phosphoryl transfer and DNA and RNA conformations. Topics include helix-coil transitions, sequence dependence of double-stranded DNA conformations, dynamic behavior of RNA and DNA, biological and genetic significance, and stereochemical problems studied with chiral thiophosphates. The conformation and dynamics of nucleic acids and phosphoproteins, relaxation behavior of nucleic acids, and theory and applications of solid-state phosphorus-31 NMR to nucleic acids are also discussed. The selection is a valuable reference for readers interested in the principles and applications of phosphorus-31 nuclear magnetic resonance.
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Table of Contents

Contributors. Preface. Introduction, D.G. Gorstein. Phosphorus-31 Chemical Shifts and Spin-Spin Coupling Constants: Principles and Empirical Observations: Phosphorus-31 Chemical Shifts: Principles and Empirical Observations, D.G. Gorenstein. Phosphorous-31 Spin-Spin Coupling Constants: Principles and Applications, D.G. Gorenstein. Theory and Applications of Phosphorus-31 NMR to Biochemistry: Phosphorus-31 NMR of Enzyme Complexes, B.D. Nageswara Rao. 31P-NMR Studies of Phosphoproteins, H.J. Vogel. Paramagnetic Probes of Enzyme Complexes with Phosphorus-Containing Compounds, J.J. Villafranca. Use of Chiral Thiophosphates and the Stereochemistry of Enzymatic Phosphoryl Transfer, M.-D. Tsai. Use of Chiral [16O, 17O, 18O] Phosphate Esters to Determine the Stereochemical Course of Enzymatic Phosphoryl Transfer Reactions, J.A. Gerlt. DNA and RNA Conformations, C.-W. Chen and J.S. Cohen. High-Resolution 31P-NMR Spectroscopy of Transfer Ribonuclei Acids, D.G. Gorenstein. Phosphorus-31 NMR of Drug-Nucleic Acid Complexes, D.G. Gorenstein and E.M. Goldfield. Phosphorus Relaxation Methods: Conformation and Dynamics of Nucleic Acids and Phosphoproteins, P.A. Hart. Relaxation Behavior of Nucleic Acids: Dynamics and Structure, T.L. James. Solid-State Phosphorus-31 NMR: Theory and Application to Nucleic Acids, H. Shindo. Phosphorus-31 NMR of Phospholipids in Micelles, E.A. Dennis and A. Pluckthun. Phosphorus-31 NMR of Phospholipids in Membranes, I.C.P. Smith and I.H. Ekiel. Application to Biology and Medicine: Future Directions: Two-Dimensional Phosphorus-31 NMR, W.C. Hutton. Identification of Diseased States by Phosphorus-31 NMR, M. Barany and T. Glonek . Selected Data: Appendixes: Selected Compilation of 31P-NMR Data, D.G. Gorenstein and D.O. Shah. Appendix I: Doubly Connected Phosphorus. Appendix II: Triply Connected Phosphorus. Appendix III: Quadruply Connected Phosphorus. Appendix IV: Quintuply Connected Phosphorus. Appendix V: Sextuply Connected Phosphorus. Index.

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