Table of Contents

Estimating RNA Secondary Structure Folding Free Energy Changes with efn2.- VarGibbs Usage in the Optimization of Nearest-Neighbor Parameters and Prediction of Melting Temperature of RNA Duplexes.- RNA Secondary Structure Thermodynamics.- RNA Secondary Structure Modeling following the IPANEMAP Workflow.- How Parameters Influence SHAPE-Directed Predictions.- Classified Dynamic Programming in RNA Structure Analysis.- Classification and Identification of Non-Canonical Base Pairs and Structural Motifs.- Modified Nucleotides and RNA Structure Prediction.- How to do RNA-RNA Interaction Prediction? A Use-Case Driven Handbook using IntaRNA.- LocARNA 2.0: Versatile Simultaneous Alignment and Folding of RNAs.- Evolutionary Structure Conservation and Covariance Scores.- Developing Complex RNA Design Applications in the Infrared Framework.- A Guide to Computational Co-Transcriptional Folding featuring the SRP RNA.- Assessing theQuality of Co-Transcriptional Folding Simulations.- The Multiscale Ernwin/SPQR RNA Structure Prediction Pipeline.