Studies Upon the Cerebral Cortex in the Normal Human Brain and in Dementia Paralytica
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|Format: ||Paperback, 132 pages|
|Published In: ||United States, 31 July 2016|
Excerpt from Studies Upon the Cerebral Cortex in the Normal Human Brain and in Dementia Paralytica
These structures had been previously observed by Flemming and Benda but by less perfect methods. Nissl at first stained tissues hardened in' alcohol with magenta red or meth ylene blue, and' cleared in oil oi' origanum. This has been modi fied in several ways, so that at present his treatment is as follows. Small blocks of tissue are hardened in 96 per cent alcohol and fastened by Weigert's method with gum arabic, without imbed ding. The sections are received in 96 per cent alcohol and stained in a watch glass. The stain is to be heated over the spirit ﬂame until small bubbles arise which make a crackling noise (65 to 70 C.) sections are then transferred to aniline-oil-alcohol until differentiated. The process of differentiation is ended. When no more coarse clouds of color go off into the ﬂuid. The section is then transferred to the slide, dried with filter paper, after which some drops of oil of cajeput are applied; and the sections are again blotted with filter paper. A few drops of benzene are poured on the sections, blotted again, and finally some benzene colophonium added, a cover glass placed over the section, and the slide heated until all the benzene gas has been driven off. Upon cooling, the section is thus permanently mounted.
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22.86 x 15.24 x 0.71 centimetres (0.19 kg)|
15+ years |