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Techniques and Methodological Approaches in Breast Cancer Research

This volume explores the myriad of techniques and methodological approaches that are being used in breast cancer research. The authors critically evaluate of the advantages and disadvantages of current methodologies, starting with the tools available for understanding the architecture of the human breast, including its tissue and cellular composition. The volume discusses the importance of functional studies in breast cancer research, especially with the help of laser capture microdissection, which allows the separation of small amounts of tissue, as well as specific cells, for biochemical analysis. In addition, the authors address methodologies including stem cell separation, which has helped in significantly understanding their role in normal breast development, but also further the understanding of breast cancer and its therapeutic management. The use of in vitro techniques and established cell lines for mechanistic studies in chemotherapeutic approaches have been invaluable will be discussed. Imaging techniques for evaluating in vitro and in vivo behavior of normal and cancerous breast tissue will be explored, as it provides a better understanding of the physiopathology of cancer. The volume will also discuss the molecular analysis of gene function in breast cancer through the transcriptomic and epigenomic profile. More importantly, the advancement of more refined techniques in sequencing will be covered. This monograph will be a comprehensive, authoritative and timely, as it addresses the emerging approaches used in breast cancer research.
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Table of Contents

Laser Capture Microdissection as a Tool for Compartmentalization Studies.- Histological Evaluation of the Normal Breast.- Methodology for Studying the Compartments of the Human Breast.- Methodological approach to Tissue Micro Array for Studying the Normal and Cancerous Human Breast.- In Vitro Techniques for Studying the Normal Breast and the use of Cell Lines in Breast Cancer Research.- In situ Methods for Indentifying the Stem Cell of the Normal and Cancerous Breast.- Imaging Techniques for Evaluation in vitro Behavior of Normal and Cancerous Breast Tissue.- In vivo Studies of Breast Cancer Cells.- RNA Sequencing in the Human Breast.- Methodological Approaches for the Understanding the Epigenetic Landscape of the of the Human Breast and its Implications in Cancer and Prevention.

About the Author

Jose Russo, M.D., FACP is a Professor and Senior Member for the Fox Chase Cancer Center. He is also the Director of the Breast Cancer and Environment Research Center at Fox Chase .In addition to his roles at Fox Chase, he is simultaneously an Adjunct Professor of Pathology and Cell Biology at Jefferson Medical College, as well as an Adjunct Professor of Biochemistry at Temple Medical School. His primary research interest includes breast cancer, endocrinology, hormonal and environmental carcinogenesis. He's received several academic and research awards for his outstanding work in the field. He's a member of numerous scientific and professional societies. He has published over 350 scientific papers. He also serves on several editorial boards for several journals including, International Journal of Oncology, Hormone Molecular Biology and Clinical Investigation, and the Journal of Medical Hypothesis and Research. In addition, he's the editor-in-chief of the Journal of Women's Cancer. He's also published several books with Springer in the past. Irma Russo, M.D., FACP, FASCP is Associate Professor at the Fox Chase Cancer Center and the Chief of the Molecular Endocrinology Section oat the Breast Cancer Research Lab. She has over 190 scientific publications.

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