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Therapeutic Applications of Ribozymes
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Expert laboratory scientists describe in detail their methodologies for constructing ribozymes designed to elucidate the role of specific genes as key routes to the development of novel therapies for a wide variety of diseases. The authors review the many sites targeted with ribozymes in various diseases and provide specific accounts of the practical techniques required for the proper use of ribozymes in these systems. Their cutting-edge protocols demonstrate how to achieve ribozyme expression in distinct cellular systems, the preparation and use of high-efficiency ribozyme DNA or RNA delivery, and the studies required to prove the efficacy of ribozyme-mediated inhibition. "Therapeutic Applications of Ribozymes" contains all the technical support needed to make ribozyme technology a widely used and significant methodology.
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Table of Contents

Small Efficient Hammerhead Ribozymes Philip Hendry, Trevor J. Lockett, and Maxine J. McCall The Hairpin Ribozyme: Discovery and Development for Gene Therapy Arnold Hampel Molecular Manipulations of the Catalytic RNAs from the Human Hepatitis Delta Virus N. Kyle Tanner The Design, Production, and Validation of an Anti-HIV Type 1 Ribozyme Lun-Quan Sun, Wayne Gerlach, and Geoff Symonds Methods for Treating HIV by Gene Therapy Using Ribozymes Eric M. Poeschla, Mang Yu, Mark C. Leavitt, and Flossie Wong-Staal An Expression Vector for Multiple Ribozymes Jun Ohkawa, Yutaka Takebe, and Kazunari Taira Viral RNA Targets for Ribozymes: Influenza A Virus Gerd Hobom and Annette Menke Hammerhead Ribozyme-Mediated Cleavage of Hepatitis B Virus RNA Fritz von Weizsacker, Hubert E. Blum, and Jack R. Wands Bovine Leukemia Virus Ribozymes Glenn H. Cantor Use of Ribozymes to Specifically Alter the MDR-1 Phenotype David Y. Bouffard Adenoviral-Mediated Anti-H-ras Ribozyme for the Treatment of Bladder Cancer Akira Irie, Jerry Tsai, and Mohammed Kashani-Sabet Tissue-Specific Delivery of an Anti-H-ras Ribozyme Against Malignant Melanoma Tsukasa Ohkawa and Mohammed Kashani-Sabet Ribozymes as a Novel Approach for the Treatment of Human Pancreatic Carcinoma Hiroshi Kijima, David Y. Bouffard, and Kevin J. Scanlon K-ras Ribozyme for Lung Cancer Alex W. Tong, Yu-An Zhang, John Nemunaitis, and Gabriele Mues Anti-c-erb-B-2 Ribozyme for Breast Cancer Toshiya Suzuki, Lisa D. Curcio, Jerry Tsai, and Mohammed Kashani-Sabet Ribozymes: Applications in Cancer Metastasis Research Gunhild M. Maelandsmo, Inge Kjonniksen, Eivind Hovig, Kevin J. Scanlon, and Oystein Fodstad Preformed Ribozymes: Strategies for Design and In Vivo Applicationas Anticytokine Agents Mouldy Sioud Folate-Polylysine-Mediated Delivery of a Multiunit Anti-BCR/ABL Ribozyme to BCR/ABL-Transformed 32D Cells Lance H. Leopold, Scott K. Shore, Ramana M. V. Reddy, and E. Premkumar Reddy Ribozymes Targeted Against AML1/MTG8: Evaluation of Ribozyme Efficiency in a Cell-Free System and Transfection with Cationic Liposome Hiromichi Matsushita, Hiroyuki Kobayashi, Masahiro Kizaki, and Yasuo Ikeda Ribozyme Targeting of the Growth Factor Pleiotrophin Frank Czubayko, Anke M. Schulte, and Anton Wellstein Ribozyme-Mediated Inhibition of Cell Proliferation: A Model for Identifying and Refining a Therapeutic Ribozyme Thale C. Jarvis, Dennis Macejak, and Larry Couture Inhibition of bAPP Synthesis by Ribozymes and Oligonucleotides in a Coupled Reticulocyte Transcription Translation System Marilyn Smedman and Robert B. Denman Selective Modification of Fatty Acid Synthesis Pathway by Expression of an Acetyl-CoA Carboxylase-Specific Ribozyme Gene Joohun Ha In Vivo Knockout of a Tissue-Specific Gene by Synthetic Ribozymes S. Petter Lyngstadaas, Brian S. Sproat, Martina Blaschke, Janos Ludwig, Thomas Rupp, and Hans P. Prydz Ribozyme-Mediated Downregulation of Gene Expression in Transgenic Mice Shimon Efrat Targeted Cleavage of RNA Using External Guide Sequences and Eukaryotic RNase P Yan Yuan and Fenyong Liu Application of In Vitro Selection in Design of External Guide Sequences That Direct Efficient Cleavage of RNA by RNase P Yan Yuan A PCR-Based Approach to Hammerhead Ribozyme Synthesis Katalin Kariko and Elliot S. Barnathan Measurement of Ribozyme Activity Using RNase Protection P. Kamath and E. J. Shillitoe Index

Reviews

". . . an impressive collection of ribozyme applications. . .a useful investment for anyone who contemplates or is actively using ribozymes for target RNA inactivations."-Nature Medicine

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