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Thyroid Hormone Receptors
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A panel of outstanding investigators surveys and explains the major cutting-edge methods used in thryroid receptor (TR) research and explains their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TR in development and knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and in living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for studying the oncogene v-Erba in blood cell differentiation, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver.
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Table of Contents

Introduction to Thyroid Hormone Receptors Aria Baniahmad Null Mutant Mice for Thyroid Hormone Receptors Karine Gauthier, Denise Aubert, Olivier Chassande, Frederic Flamant, and Jacques Samarut Transgenic Targeting of a Dominant Negative Corepressor to Liver and Analyses by cDNA Microarray Xu Feng, Paul Meltzer, and Paul M. Yen The Role of Thyroid Hormone Receptors in the Heart Wolfgang H. Dillmann and Bernd R. Gloss Analysis of Thyroid Hormone-Dependent Genes in the Brain by In Situ Hybridization Juan Bernal and Ana Guadano-Ferraz. The v-erbA Oncogene: Assessing Its Differentiation-Blocking Ability Using Normal Chicken Erythrocytic Progenitor Cells, Olivier Gandrillon Gene Regulation by Thyroid Hormone Receptors Helmut Dotzlaw and Aria Baniahmad Molecular Analysis of Human Resistance to Thyroid Hormone Syndrome Sunnie M. Yoh and Martin L. Privalsky Function of Thyroid Hormone Receptors During Amphibian Development Sashko Damjanovski, Laurent M. Sachs, and Yun-Bo Shi Transcriptional Regulation by Thyroid Hormone Receptor in Chromatin Jiemin Wong Gene Activation by Thyroid Hormone Receptor In Vitro and Purification of the TRAP Coactivator Complex Joseph D. Fondell Index

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