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Fluorescence spectroscopy continues its advance to more sophisticated methods and applications. As one looks over the previous decades, its appears that the first practical instruments for time-resolved measurements appeared in the 1970's. The instrumentation and analysis methods for time-resolved fluorescence advanced rapidly throughout the 1980's. Since 1990 we have witnessed a rapid migration of the principles of time-resolved fluorescence to cell biology and clinical appli- tions. Most recently, we have seen the introduction of multi-photon excitation, pump-probe and stimulated emission methods for studies of biological mac- molecules and for cellular imaging. These advanced topics are the subject of the present volume. Two-photon excitation was first predicted by Maria Goppert-Mayer in 1931, but was not experimentally observed until 1961. Observation of two-photon excitation required the introduction of lasers which provided adequate photon density for multi-photon absorption. Since the early observations of two-photon excitation in the 1960s, multi-photon spectroscopy has been limited to somewhat exotic applications of chemical physics, where it is used to study the electronic symmetry of small molecules. Placing one's self back in 1980, it would be hard to imagine the use of multi-photon excitation in biophysics or cellular imaging.
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Table of Contents

The Theory of Two-Photon Induced Fluorescence Anisotropy;P.R. Callis. Anisotropy Decays Induced by Two-Photon Excitation; C.K. Johnson, C. Wan. Multiphoton Excitation of Biochemical Fluorophores; J.R. Lakowicz, I. Gryczynski. Two-Photon Excitation and Anisotropy Decays in Membranes and Oriented Systems; W. van der Meer, S.-Y.S. Chen. Two-Photon Induced Fluorescence of Proteins; b. Kierdaszuk, et al. Fluorescence and Multiwave Mixing Induced by Photon Absorption of Excited Molecules; V. Bogdanov. Ultrafast Stimulated Emission Spectroscopy; G.J. Blanchard. Fluorescence Quenching by Stimulated Emission; J.R. Lakowicz, I. Gryczynski. Increasing the Resolution of Far-Field Fluorescence Light Microscopy by Point Spread Functional Engineering; S.W. Hell. Time-Resolved Stimulated-Emission and Transient-Absorption Microscopy and Spectroscopy; P.T.C. So, et al. Index.

About the Author

Dr. J.R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is the sole author of the widely used text, Principles of Fluorescence Spectroscopy, also published by Kluwer Academic/Plenum Publishers, now in its Second Edition.


`A wealth of information..indispensable for researchers in the life sciences, should also be consulted by analytical chemists.` Trends in Analytical Chemistry `Commendable ... the reference volumes will enhance the collection of the fluorescence spectroscopist.' Applied Spectroscopy `The [editor] has mastered a commendable task; the topics appear to be well covered, and the volumes will enhance the collection of the fluorescence spectroscopist.' Applied Spectroscopy

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