* Basic Methods in Yeast Genetics
* Physical and genetic mapping
* Making and recovering mutants
* Cloning and Recombinant DNA Methods
* High-efficiency transformation
* Preparation of yeast artificial chromosome vectors
* Basic Methods of Cell Biology
* Immunomicroscopy
* Protein targeting assays
* Biochemistry of Gene Expression
* Vectors for regulated expression
* Isolation of labeled and unlabeled DNA, RNA, and protein
Basic Methods of Yeast Genetics:
F. Sherman, Getting Started with Yeast.
F. Sherman and J. Hicks, Micromanipulation and Dissection of
Asci.
F. Sherman and P. Wakem, Mapping Yeast Genes.
S.L. Gerring, C. Connelly, and P. Hieter, Positional Mapping of
Genes by Chromosome Blotting and Chromosome Fragmentation.
G.F. Sprague, Jr., Assay of Yeast Mating Reaction.
Y. Kassir and G. Simchen, Monitoring Meiosis and Sporulation in
Saccharomyces cerevisiae.
R.E. Esposito, M. Dresser, and M. Breitenbach, Identifying
Sporulation Genes, Visualizing Synaptonemal Complexes, and
Large-Scale Spore and Spore Wall Purification.
I. Herskowitz and R.E. Jensen, Putting the HO Gene to Work:
Practical Uses for Mating-Type Switching.
B. Rockmill, E.J. Lambie, and G.S. Roeder, Spore Enrichment.
T.D. Fox, L.S. Folley, J.J. Mulero, T.W. McMullin, P.E. Thorsness,
L.O. Hedin, and M.C. Costanzo, Analysis and Manipulation of Yeast
Mitochondrial Genes.
Cloning and Recombinant DNA:
P. Philippsen, A. Stotz, and C. Scherf, DNA of Saccharomyces
cerevisiae.
D.M. Becker and L. Guarente, High-Efficiency Transformation of
Yeast by Electroporation.
J.A. Heinemann and G.F. Sprague, Jr., Transmission of Plasmid DNA
to Yeast by Conjugation with Bacteria.
M.D. Rose and J.R. Broach, Cloning Genes by Complementation in
Yeast.
R.A. Young and R.W. Davis, Gene Isolation with ~glgt11 System.
J. Rine, Gene Overexpression in Studies of Saccharomyces
cerevisiae.
D.T. Burke and M.V. Olson, Preparation of Clone Libraries in Yeast
Artificial-Chromosome Vectors.
Making Mutants:
C.W. Lawrence, Classical Mutagenesis Techniques.
R. Rothstein, Targeting, Disruption, Replacement, and Allele
Rescue. Integrative DNA Transformation in Yeast.
R.S. Sikorski and J.D. Boeke, In Vitro Mutagenesis and Plasmid
Shuffling.
From Cloned Gene to Mutant Yeast:
J.N. Strathern and D.R. Higgins, Recovery of Plasmids from Yeast
into Escherichia coli.
M.F. Hoekstra, Shuttle Vectors.
H.S. Seifert, J. Nickoloff, and F. Heffron, Shuttle Mutagenesis.
Bacterial Transposons for Genetic Manipulations in Yeast:
D.J. Garfinkel and J.N. Strathern, Ty Mutagenesis in Saccharomyces
cerevisiae.
R.P. Moerschell, G. Das, and F. Sherman, Transformation of Yeast
Directly with Synthetic Oligonucleotides.
Biochemistry of Gene Expression:
J.C. Schneider and L. Guarente, Vectors for Expression of Cloned
Genes in Yeast.
Regulation, Overproduction, and Underproduction:
M. Schena, D. Picard, and K.R. Yamamoto, Vectors for Constitutive
and Inducible Gene Expression in Yeast.
K. Kadohrer and H. Domdey, Preparation of High Molecular Weight
RNA.
J.A. Wise, Preparation and Analysis of Low Molecular Weight RNAs
and Small Ribonucleoproteins.
R. Parker, D. Herrick, S.W. Peltz, and A. Jacobson, Measurement of
mRNA Decay Rates in Saccharomyces cerevisiae
J.R. Warner, Labeling of RNA and Phosphoproteins in Saccharomyces
cerevisiae.
E.W. Jones, Tackling the Protease Problem in Saccharomyces
cerevisiae.
H.A. Rauaae, W.H. Mager, and R.J. Planta, Structural and Functional
Analysis of Yeast Ribosomal Proteins.
T.J. Koerner, J.E. Hill, A.M. Myers, and A. Tzagoloff,
High-Expression Vectors with Multiple Cloning Sites for
Construction of trpE Fusion Genes: pATH Vectors.
D.T. Moir and L.S. Davidow, Production of Proteins by Secretion
from Yeast.
P.A. Kolodziej and R.A. Young, Epitope Tagging and Protein
Surveillance.
K. Struhl, Reverse Biochemistry: Methods and Applications for
Synthesizing Yeast Proteins in Vitro
M.J. Leibowitz, F.P. Barbone, and D.E. Georgopoulos, In Vitro
Protein Synthesis.
N.F. Lue, P.M. Flanagan, R.J. Kelleher III, A.M. Edwards, and R.D.
Kornberg, RNA Polymerase II Transcription in Vitro.
J.M. Huibregtse and D.R. Engelke, Direct Sequence and Footprint
Analysis of Yeast DNA by Primer Extension.
Cell Biology:
J.R. Pringle, A.E.M. Adams, D.G. Drubin, and B.K. Haarer,
Immunofluorescence Methods for Yeast.
B. Byers and L. Goetsch, Preparation of Yeast Cells for
Thin-Section Electron Microscopy.
M.W. Clark, Immunogold Labeling of Yeast Ultrathin Sections.
M.P. Yaffe, Analysis of Mitochondrial Function and Assembly.
C.J. Roberts, C.K. Raymond, C.T. Yamashiro, and T.H. Stevens,
Methods for Studying the Yeast Vacuole.
A. Franzusoff, J. Rothblatt, and R. Schekman, Analysis of
Polypeptide Transit through Yeast Secretory Pathway.
P.D. Garcia, W. Hansen, and P. Walter, In Vitro Protein
Translocation across Microsomal Membranes of Saccharomyces
cerevisiae.
P. Orlean, M.J. Kuranda, and C.F. Albright, Analysis of
Glycoproteins from Saccharomyces cerevisiae.
V. Dulic, M. Egerton, I. Elguindi, S. Raths, B. Singer, and H.
Riezman, Yeast Endocytosis Assays.
C.M. Nicolet and E.A. Craig, Inducing and Assaying Heat-Shock
Response in Saccharomyces cerevisiae.
M.W. Clark, Nucleolar-Specific Positive Stains for Optical and
Electron Microscopy.
A.E.M. Adams and J.R. Pringle, Staining of Actin with
Fluorochrome-Conjugated Phalloidin.
J.R. Pringle, Staining of Bud Scars and Other Cell Wall Chitin with
Calcofluor.
J.P. Aris and G. Blobel, Isolation of Yeast Nuclei.
J.H. Shero, M. Koval, F. Spencer, R.E. Palmer, P. Hieter, and D.
Koshland, Analysis of Chromosome Segregation in Saccharomyces
cerevisiae.
V. Berlin, J.A. Brill, J. Trueheart, J.D. Boeke, and G.R. Fink,
Genetic Screens and Selections for Cell and Nuclear Fusion
Mutants.
Fission Yeast:
S. Moreno, A. Klar, and P. Nurse, Molecular Genetic Analysis of
Fission Yeast Schizosaccharomyces pombe.
Appendix:
R.K. Mortimer, D. Schild, C.R. Contopoulou, and J.A. Kans, Genetic
and Physical Maps of Saccharomyces cerevisiae.
Each chapter includes references.
Author Index.
Subject Index.
Edited by Christine Guthrie, Gerald R. Fink, John N. Abelson and Melvin I. Simon
from:Praise for the Volume
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current practitioners. It should enable newcomers to set up a yeast
laboratory and master basic manipulations." --CAMBRIDGE SCIENTIFIC
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proclamation that yeast biology is now in the main stream." --ASM
NEWS
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