Table of Contents

Introduction: Principles of Electron Tomography.- Sample Shrinkage and Radiation Damage of Plastic Sections.- Electron Tomography of Frozen-hydrated Sections of Cells and Tissues.- The Electron Microscope as a Structure Projector.- Cryotomography: Low-dose Automated Tomography of Frozen-hydrated Specimens.- Fiducial Marker and Hybrid Alignment Methods for Single- and Double-axis Tomography.- Markerless Alignment in Electron Tomography.- Algorithms for Three-dimensional Reconstruction From the Imperfect Projection Data Provided by Electron Microscopy.- Weighted Back-projection Methods.- Reconstruction With Orthogonal Functions.- Resolution in Electron Tomography.- Denoising of Electron Tomograms.- Segmentation of Three-dimensional Electron Tomographic Images.- Segmentation of Cell Components Using Prior Knowledge.- Motif Search in Electron Tomography.- Localization and Classification of Repetitive Structures in Electron Tomograms of Paracrystalline Assemblies.