1 The Protein Purification Laboratory.- 2 Making an Extract.- 3 Analysis—Measurement of Protein and Enzyme Activity.- 4 Separation by Precipitation.- 5 Separation by Adsorption I: General Principles.- 6 Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents.- 7 Separation by Adsorption—Affinity Techniques.- 8 Separation in Solution.- 9 Purification of Special Types of Proteins.- 10 Small-Scale and Large-Scale Procedures.- 11 Analysis for Purity.- 12 Optimization of Procedures; Final Steps.- Appendix A.- Precipitation Tables.- Appendix B.- Solutions for Measuring Protein Concentration.- Appendix C.- Buffers for Use in Protein Chemistry.- Appendix D.- Chromatographic Materials.- References.
A classic guide to protein purification
From reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly" (American Scientist), "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature)
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