Table of Contents

Concepts.- Protein Misfolding and Cellular Stress: An Overview.- Protein Aggregation Diseases: Toxicity of Soluble Prefibrillar Aggregates and Their Clinical Significance.- Consequences of Stress in the Secretary Pathway: The ER Stress Response and Its Role in the Metabolic Syndrome.- What Role Does Mitochondrial Stress Play in Neurodegenerative Diseases?.- Autophagy in Disease.- Mitophagy and Mitoptosis in Disease Processes.- Cellular Stress and Protein Misfolding During Aging.- Measuring Consequences of Protein Misfolding and Cellular Stress Using OMICS Techniques.- Protocols.- Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable and Regulated Expression Using the Flp Recombinase System.- Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress.- Examining Ubiquitinated Protein Aggregates in Tissue Sections.- Determination of Proteasomal Activities.- Measurement of Autophagy in Cells and Tissues.- Transcription Factor Sequestration by Polyglutamine Proteins.- Biological Membranes as Protein Aggregation Matrices and Targets of Amyloid Toxicity.- Measurement of Mitochondrial ROS Production.- In Vivo Detection of Oxidized Proteins: A Practical Approach to Tissue-Derived Mitochondria.- Measurement of Oxidized/Reduced Glutathione Ratio.- Determination of Altered Mitochondria Ultrastructure by Electron Microscopy.- Assessing Bad Sub-cellular Localization Under Conditions Associated with Prevention or Promotion of Mitochondrial Permeability Transition-Dependent Toxicity.- A Cellular Viability Assay to Monitor Drug Toxicity.- Rescue of Misfolded Proteins and Stabilization by Small Molecules.

Reviews

From the reviews:“Methods in Molecular Biology deals with current topics and techniques in cellular stress as well as methods to study and analyze the effects of cellular stress on protein misfolding. … The book is written mainly for scientists involved in the study of diseases resulting from protein misfolding. … the protocol is straightforward and is based on proven examples that render these diverse methods credible. This should prove to be a valuable companion to all researchers in the field of cellular stress and protein misfolding.” (Ruben Mestril, Doody’s Review Service, February, 2011)“Drs. Peter Bross e Niels Gregersen … invited scientists who are experts in Protein Misfolding to collaborate in the book’s edition … . focuses in the main points of the latest literature on Protein Misfolding on genesis of diseases and aging processes, and also provides experimental and analytical protocols employed to protein misfolding detection. … presented in two parts: the first one comprehends protein misfolding concepts, their importance on disease genesis, and the second one, describe experimental protocols employed in the investigation of intracellular alterations.” (Sandra Helena P. Farsky, Brazilian Journal of Pharmaceutical Sciences, Vol. 47 (2), Summer, 2011)“This new volume constitutes an excellent opportunity to be driven on the causes and mechanisms that are actually know to produce the misfolding. … The concepts and protocols here presented are a good example of what the scientific community refers as molecular medicine: there is no need to change the academic curricula to teach the molecular bases of present day medicine, simply you have to well present on which physiological parameters the future doctor visit will sounds!” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)