Table of Contents

 Rapid Generation of Dityrosine Cross-linked Aβ Oligomers via Cu-redox Cycling.-Application of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic Proteins.-PREPARATION OF STABLE AMYLOID β-PROTEIN OLIGOMERS OF DEFINED ASSEMBLY ORDER.-Purification and Fibrillation of full-Length Recombinant PrP.-Featuring Amyloids with Fourier Transform Infrared and Circular Dichroism Spectroscopies .-Quasielastic Light Scattering Study of Amyloid b -Protein Fibrillogenesis.-Conformations of Microtubule-Associated Protein Tau  Mapped by Fluorescence Resonance Energy Transfer (FRET).-Measuring the Kinetics of Amyloid Fibril Elongation using Quartz Crystal Microbalances (QCM).-X-ray Fibre Diffraction Studies of Amyloid Fibrils.-Structural Characterization of Pre-fibrillar Intermediates and Amyloid Fibrils by Small-angle X-ray Scattering.-Atomic Force Fluorescence Microscopy in the Characterization of Amyloid Fibril Assembly and Oligomeric Intermediates.Investigating Fibrillar Aggregates of Tau Protein by Atomic Force Microscopy.-Structural Studies of Amyloids by Quenched Hydrogen-Deuterium Exchange by NMR.-Cyclic Amplification of Prion Protein Misfolding .-Search for Amyloid Binding Proteins by Affinity Chromatography.-Using Biophysical Approaches for Establishing the Links Between Aβ Aggregation and Cytotoxicity in Vitro.-Preparation of Cultured Human Vascular Cells.-Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy: Isolation of Smooth Muscle and Endothelial Cells from Mouse Brain.-IN VITROASSAYS MEASURING PROTECTION BY PROTEINS SUCH AS CYSTATIN C OF PRIMARY CORTICAL NEURONAL AND SMOOTH MUSCLE CELLS.-Study of Neurotoxic Intracellular Calcium Signalling Triggered by Amyloids.-Bacterial Amyloids.-Study of Amyloids using Yeast.-Cell-to-cell Transmission of a-synuclein Aggregate.-Subcutaneous Adipose Tissue Biopsy for Amyloid Protein Studies.-Analysis of S100 Oligomers and Amyloids.-S100A8/A9 Amyloidosis in the Ageing Prostate: Relating ex vivo and in vitro Studies.-Isolation of Amyloid by Solubilization in Water.-Histological Staining of Amyloid and Pre-Amyloid Peptides and Proteins in Mouse Tissue.-A Pentameric Luminescent Conjugated Oligothiophene for Optical Imaging of in vitro Formed Amyloid Fibrils and Protein Aggregates in Tissue Sections.-In Vivo Magnetic Resonance Imaging of Amyloid-β Plaques in Mice.-The Mouse Model for Scrapie: Inoculation, Clinical Scoring and Histopathological Techniques.-Biochemical Isolation of Insoluble Tau in Transgenic Mouse Models of Tauopathies.-Tissue Processing Prior to Analysis of Alzheimer’s Disease Associated Proteins and Metabolites, Including Ab.-Ab Measurement by Enzyme-linked Immunosorbent Assay (ELISA).-Cognitive and Sensorimotor Tasks for Assessing Functional Impairments in Mouse Models of Alzheimer’s Disease and Related Disorders.