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Table of Contents

Analytical Techniques.- Two-Dimensional Electrophoresis of Membrane Proteins.- Analysis of Membrane Antigens by Means of Quantitative Detergent- Immunoelectrophoresis(With 3 Figures and 7 Plans).- Two-Dimensional Thin Layer Chromatography. Separation of Lipids Extracted from Native and Phospholipase A2 Treated Human Erythrocyte Ghosts (With 1 Figure).- Separation of Fatty Acids from Plant Membrane Lipids by a Combined GLC-TLC Procedure.- Isolation of Proteins.- Affinity-Chromatography of Mouse Histocompatibility Antigens on Lens culinaris Lectin-Sepharose (With 3 Figures).- Isolation of a Membrane Protein: The Phospholipase A2 from Sheep Erythrocyte Membranes.- Isolation and Purification of the Nicotinic Acetylcholine Receptor from Torpedo Electric Organ.- Preparation of Spectrin-Free Vesicles from Human Red Blood Cells (With 5 Figures).- Purification by Affinity Chromatography of Red Cell Membrane Acetylcholinesterase (With 5 Figures).- Purification of Cytochrome c Oxidase by Affinity Chromatography.- Reconstitution.- Reconstitution of Pure Acetylcholine Receptor (With 2 Figures).- Reconstitution and Assay of Mitochondrial ADP/ATP Transport with a Partially Purified ADP/ATP Carrier Protein (With 3 Figures).- Investigations on the Purple Membrane of Halobacterium halobium (With 2 Figures).- Enzymology of Succinate: Ubiquinone Reductase in Detergent Solution and Reconstituted Membranes.- Isolation and Reconstitution of Band 3, the Anion Transporter of the Human Red Blood Cell Membrane (With 3 Figures).- Modification.- Chemical Modification of a Membrane Protein by Hydrophobic and Hydrophilic Labeling Reagents (With 2 Figures).- Crosslinking of an Amphiphilic Protein: Human Erythrocyte Membrane Acetylcholinesterase (With 2 Figures).- Labeling of Membranes from Within the Lipid Core: Use of a Photo- activatable Carbene Generator (With 2 Figures).- Characterization — Spectral Techniques.- Applications of Spin-Labels to Biological Systems (With 14 Figures).- The Application of Fluorescence Spectroscopy to the Study of Biological Membranes (With 2 Figures).- Use of Potentiometric Cyanine Dye in the Study of Reconstituted Membrane Proteins (With 3 Figures).- CD Measurements to Probe the Structure of the Purple Membrane (With 5 Figures).- Characterization — Other Techniques.- Lipid-Protein Interactions in Monolayers at the Air-Water Interface (With 7 Figures).- Incorporation of the Polypeptide Hormone ACTH into Planar Lipid Bilayers: Evaluation by the Capacitance Minimization Technique (With 6 Figures).- Demonstration of Liposomes by Electron Microscopy (With 6 Figures).