In this volume we have brought together a number of core protocols concentrating on DNA, carefully written and edited by experts.
Explanatory chapter: PCR Primer design
Explanatory Chapter: How Plasmid Preparation Kits Work
Explanatory Chapter: Introducing Exogenous DNA into cells
Agarose Gel Electrophoresis
Analysis of DNA by Southern Blotting
Purification of DNA Oligos by Denaturing Polyacrylamide Gel
Electrophoresis (PAGE)
Molecular Cloning
Rapid creation of stable mammalian cell lines for regulated
expression of proteins using the Gateway® Recombination Cloning
Technology and Flp-In T-REx® lines
Restrictionless cloning
Isolation of plasmid DNA from bacteria
Preparation of Genomic DNA from Bacteria
Preparation of Genomic DNA from Saccharomyces cerevisiae
Isolation of Genomic DNA from Mammalian Cells
Sanger Dideoxy Sequencing of DNA
Preparation of fragment libraries for Next-Generation Sequencing on
the Applied Biosystems SOLiD platform
Explanatory Chapter: Next Generation Sequencing
Generating mammalian stable cell lines by electroporation
Transient mammalian cell Transfection with Polyethylenimine
(PEI)
Site-Directed Mutagenesis
PCR-based random mutagenesis
Megaprimer Method for Mutagenesis of DNA
Explanatory Chapter: Troubleshooting PCR
Explanatory Chapter: Quantitative PCR
General PCR
Colony PCR
Chemical Transformation of Yeast
Transformation of E. coli via electroporation
Transformation of Chemically Competent E. coli
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