Table of Contents

​Coronaviruses: An Overview of Their Replication and Pathogenesis.- Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics.- Serological Diagnosis of Feline Coronavirus Infection by Immunochromatographic Test.- Estimation of Evolutionary Dynamics and Selection Pressure in Coronaviruses.- The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration.- The Preparation of Chicken Kidney Cell Cultures for Virus Propagation.- Isolation and Propagation of Coronaviruses in Embryonated Eggs.- Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures.- Quantification of Infectious Bronchitis Coronaviruses by Titration in vitro and in ovo.- Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis.- Partial Purification of IBV and Subsequent Isolation of Viral RNA for Next Generation Sequencing.- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses.- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes.- Protein Histochemistry Using Coronaviral Spike Proteins: Studying Binding Profiles and Sialic Acid Requirements for Attachment to Tissues.- Identification of Protein Receptors for Coronaviruses by Mass Spectrometry.- Single Particle Tracking Assay to Study Coronavirus Membrane Fusion.- Studying Coronavirus-Host Protein Interactions.- A Field-Proven Yeast Two-Hybrid Protocol Used to Identify Coronavirus-Host Protein-Protein Interactions.- Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach.- Transcriptome Analysis ofFeline Infectious Peritonitis Virus (FIPV) Infection.- Quantification of Interferon Signaling in Avian Cells.- Studying the Dynamics of Coronavirus Replicative Structures.- Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope.