Model Systems to Study Mitochondrial Functions.- The Mitochondria of Cultured Mammalian Cells.- The Mitochondria of Cultured Mammalian Cells.- Drosophila melanogaster as a Model System to Study Mitochondrial Biology.- Isolation and Functional Analysis of Mitochondria From the Nematode Caenorhabditis elegans.- Isolation of Mitochondria From Procyclic Trypanosoma brucei.- Saccharomyces cerevisiae as a Model Organism to Study Mitochondrial Biology.- Studying Mitochondria in an Attractive Model: Schizosaccharomyces pombe.- Neurospora crassa as a Model Organism for Mitochondrial Biogenesis.- Isolation of Intact, Functional Mitochondria From the Model Plant Arabidopsis thaliana.- Chlamydomonas reinhardtii: The Model of Choice to Study Mitochondria From Unicellular Photosynthetic Organisms.- Genetics and Molecular Biology.- Directed Alteration of Saccharomyces cerevisiae Mitochondrial DNA by Biolistic Transformation and Homologous Recombination.- Screens for Mitochondrial Mutants in Yeast.- Transcript End Mapping and Analysis of RNA Editing in Plant Mitochondria.- In Vitro Analysis of the Yeast Mitochondrial RNA Polymerase.- Functional Analysis by Inducible RNA Interference in Drosophila melanogaster.- Analysis of Replicating Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis.- Biochemistry.- The Analysis of tRNA Import Into Mammalian Mitochondria.- Analysis of Mitochondrial Protein Synthesis in Yeast.- Large-Scale Isolation of Mitochondrial Ribosomes From Mammalian Tissues.- Protein Import Into Isolated Mitochondria.- Analyzing Import Intermediates of Mitochondrial Proteins by Blue Native Gel Electrophoresis.- In Vitro and In Vivo Protein Import Into Plant Mitochondria.- Two-Dimensional Blue Native/Blue Native Polyacrylamide Gel Electrophoresis for the Characterization of Mitochondrial Protein Complexes and Supercomplexes.- Investigation of Iron-Sulfur Protein Maturation in Eukaryotes.- Studying Proteolysis Within Mitochondria.- Physiology.- Methods to Determine the Status of Mitochondrial ATP Synthase Assembly.- ATP Production in Isolated Mitochondria of Procyclic Trypanosoma brucei.- Oxidative Stress and Plant Mitochondria.- Measuring Mitochondrial Shape Changes and Their Consequences on Mitochondrial Involvement During Apoptosis.- Assessing Mitochondrial Potential, Calcium, and Redox State in Isolated Mammalian Cells Using Confocal Microscopy.- Dynamics and Morphology.- Fluorescence Imaging of Mitochondria in Yeast.- Mitochondrial Fusion In Vitro.- Conventional and Immunoelectron Microscopy of Mitochondria.- Mitochondrial Division in Caenorhabditis elegans.- Functional Genomics.- Yeast Mitochondrial Transcriptomics.- Plant Mitochondrial Transcriptomics by Quantitative RT-PCR.- Proteomics of Yeast Mitochondria.- Arabidopsis Mitochondrial Proteomics.- MitoP2, an Integrated Database for Mitochondrial Proteins.
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