Table of Contents

Nucleic Acid Methods.- The Manipulation of Nucleic Acids.- Restriction Enzymes.- Principles and Medical Applications of the Polymerase Chain Reaction.- Probe Design, Production, and Applications.- Southern Blotting as a Diagnostic Method.- Capillary Electrophoresis of DNA.- Denaturing High-Performance Liquid Chromatography (DHPLC) for Nucleic Acid Analysis.- Denaturing Gradient Gel Electrophoresis (DGGE).- Single Strand Conformation Polymorphism (SSCP) Analysis.- Randomly Amplified Polymorphic DNA (RAPD).- Quantification of mRNA Using Real Time RT-PCR.- Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing.- Multiplex Amplifiable Probe Hybridization (MAPH).- Gene Expression Profiling.- Comparative Genomic Hybridization in Clinical and Medical Research.- Subtractive Hybridization.- Fluorescence In Situ Hybridization.- Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice.- cDNA Microarrays.- Mapping Techniques.- Single Nucleotide Polymorphisms.- Gene/Protein Sequence Analysis.- Protein and Cell Methods.- Protein Electrophoresis.- Protein Blotting.- Capillary Electrophoresis of Proteins.- Autoradiography and Fluorography.- Mass Spectrometry of Proteins and Peptides.- Post-Translational Modif ications of Proteins.- Protein Microarray Technology.- Protein–Protein Interactions.- Glycoprotein Analysis.- Solid-Phase Peptide Synthesis.- Monoclonal Antibodies.- Antibody Phage Display.- Protein Engineering.- Directed Protein Evolution.- Enzyme Linked Immunosorbent Assay (ELISA).- Epitope Mapping.- Quantum Dots.- Ion-Exchange Chromatography.- Size-Exclusion Chromatography.- Hydrophobic Interaction Chromatography.- Affinity Chromatography.- High Performance Liquid Chromatography (HPLC) of Peptides and Proteins.- Amino Acid Analysis.- Surface Plasmon Resonance.- Macromolecular Crystallography.- Microchip Devices for Bioanalysis.- Mammalian Cell Culture.- Plant Tissue Culture.- Stem Cells and Regenerative Medicine.- Cryopreservation.- Magnetic Resonance Imaging.- Electron Microscopy.- Confocal Microscopy.- Laser Microdissection.- Biomedical Uses of Flow Cytometry.- Immunomicroscopy.- In Situ Hybridization.- High Throughput Screening.

Reviews

From the reviews of the second edition: "If you want to know the theoretical basis of how a technique works, how it might be applied, or what techniques are available to allow you to ask a particular question, then this book could prove very useful. … this book will be of interest to young (and not so young) scientists who want to better understand the techniques they use, or may wish to use, and would be a useful addition to a library or PhD room." (Bob Dalziel, Microbiology Today, February, 2009) "The book is a second edition and this includes several new sections which deal with new areas e.g. microarray technology and nanotechnology … . These deal with the physical concepts behind the techniques in an accessible way and will be useful to a large number of researchers. … In summary, I found the book to be generally comprehensive, clearly written and logically organised. … it would be a useful addition to any molecular biology laboratory bookshelf." (Nicola J Stonehouse, British Toxicology Society Newsletter, June-August, 2009) “Written by experts in their fields, the book gives a wide scope of molecular biology experimentation really living out only the most specialized uncommon techniques while at the same time giving reference to each chapter and sharing full information with readers. This handbook is suitable for research scientists, technicians, and graduate research students in molecular and cell biology, proteomics, and related branches of science.” (G. Ya. Wiederschain, Biochemistry, Vol. 74 (9), 2009)